引用本文:孙磊,宋彤彤,朱珍妮,李丹阳,刘存歧,等.可降解三乙胺的赤红球菌S6-2的筛选与鉴定及降解特性[J].环境科学研究,2016,29(12):1882-1886.
SUN Lei,SONG Tongtong,ZHU Zhenni,LI Danyang,LIU Cunqi,et al.Screening,Identification and Characterization of a Triethylamine Degrading Bacterium Rhodococcus ruber S6-2[J].Reserrch of Environmental Science,2016,29(12):1882-1886.]
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 678次   下载 735 本文二维码信息
码上扫一扫!
分享到: 微信 更多
可降解三乙胺的赤红球菌S6-2的筛选与鉴定及降解特性
孙 磊1,2, 宋彤彤1, 朱珍妮1, 李丹阳1, 刘存歧1
1.河北大学生命科学学院, 河北 保定 071002 ;2.河北省微生物多样性研究与应用实验室, 河北 保定 071002
摘要:
为治理制药废水中残留的难降解有机污染物TEA(triethylamine,三乙胺),以石家庄某污水处理厂的活性污泥为材料,采用富集培养和选择培养,分离筛选到1株能以TEA为唯一碳源和氮源生长代谢的降解菌——S6-2.通过测定形态特征、生理生化特性、G+C(碱基对)摩尔百分比及16S rRNA基因序列系统发育分析,该菌株被鉴定为赤红球菌(Rhodococcus ruber).采用单一影响因素试验分析菌株S6-2对TEA的降解特性,结果表明:菌株S6-2具有较强的TEA降解能力及降解稳定性;其最适降解温度为32 ℃,最适降解pH为8.0.菌株S6-2可耐受较高浓度TEA,在ρ(TEA)为900 mg/L的培养基中仍能生长;在最适条件下,菌株S6-2对TEA的降解率为70.7%±1.8%.该株菌在含TEA的无机盐平板上传代培养15代后,对TEA的降解率为69.3%±2.5%,说明菌株S6-2对TEA的降解具有稳定性.研究显示,菌株S6-2可作为TEA污染水体生物修复的潜在资源.
关键词:  三乙胺  生物降解  赤红球菌  分离鉴定
DOI:
分类号:
基金项目:国家水体污染控制与治理科技重大专项(2012ZX07203003);河北省生物工程重点学科项目
Screening,Identification and Characterization of a Triethylamine Degrading Bacterium Rhodococcus ruber S6-2
SUN Lei1,2, SONG Tongtong1, ZHU Zhenni1, LI Danyang1, LIU Cunqi1
1.College of Life Sciences, Hebei University, Baoding 071002, China ;2.Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding 071002, China
Abstract:
Abstract: In order to treat wastewater contaminated by triethylamine, which is generated from pharmaceutical factories, bacterial strains which could degrade triethylamine were screened. A bacterial strain S6-2 that used triethylamine as the sole carbon and energy source was isolated from the activated sludge of a wastewater treatment plant in Shijiazhuang by enrichment and selective medium. S6-2 was identified as Rhodococcus ruber by morphological, physiological and biochemical characteristics, as well as (G+C)mol percentage and 16S rRNA gene phylogenic analysis. Through single-factor experiments of degrading features, S6-2 was shown to have high degradation ability and stability. The study demonstrated that temperature and pH were important factors for triethylamine degradation. The optimal temperature was 32 ℃, and the optimal pH was 8.0.This strain was tolerant to a high concentration oftriethylamine and grew normally in tradethylamine as high as 900 mg/L. Under the optimal conditions, the triethylamine degradation rate reached 70.7%±1.8%. After 15 generations subcultured on mineral salt plates supplemented with triethylamine, the triethylamine degradation rate was 69.3%±2.5%. The results showed that S6-2 had steady ability of triethylamine degradation, and suggested that S6-2 could be a potential strain for treating triethylamine-contaminated wastewater.
Key words:  triethylamine  biodegradation  Rhodococcus ruber  identification