引用本文:赵欣艳,王海燕,侯泽英,储昭升,杨永哲,等.洱海沙坪湾湖滨带不同植被带中细菌分布特征[J].环境科学研究,2017,30(5):705-711.
ZHAO Xinyan,WANG Haiyan,HOU Zeying,CHU Zhaosheng,YANG Yongzhe,et al.Bacteria Distribution Characteristics of Different Vegetation Belts along Shapingwan Riparian Zone of Erhai Lake[J].Reserrch of Environmental Science,2017,30(5):705-711.]
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 335次   下载 460 本文二维码信息
码上扫一扫!
分享到: 微信 更多
洱海沙坪湾湖滨带不同植被带中细菌分布特征
赵欣艳1,2, 王海燕2,3, 侯泽英2, 储昭升2, 杨永哲1
1.西安建筑科技大学环境与市政工程学院, 陕西 西安 710055 ;2.中国环境科学研究院, 国家环境保护湖泊污染控制重点实验室, 湖泊水污染治理与生态修复技术国家工程实验室, 北京 100012 ;3.中国环境科学研究院水污染控制技术研究中心, 北京 100012
摘要:
湖滨带是湖泊保护的重要屏障,其不同植被带中的细菌对入湖污染物降解起重要作用. 为研究不同植被带中的细菌分布,以洱海沙坪湾湖滨带为研究对象,采用Illumina HiSeq高通量测序方法,对其中AG(乔草植被带)、EP(挺水植被带)、SP(沉水植被带)和UV(无植被带)的土壤或沉积物中的细菌多样性和群落结构进行研究. 结果表明:湖滨带植被带中细菌种类丰富,共有54个门、106个纲、192个目、334个科和503个属被检测到,细菌的OTUs从高到低依次为EP(3 162个)、AG(2 926个)、UV(2 709个)和SP(2 427个),Shannon-Wiener多样性指数从高到低依次为EP(10.14)、AG(9.75)、SP(9.44)和UV(9.26);湖滨带植被带中主要细菌类群是变形菌门(Proteobacteria)(AG,48.55%;EP,47.83%;SP,40.36%;UV,44.00%)、酸杆菌门(Acidobacteria)(AG,19.30%;EP,5.31%;SP,5.67%;UV,6.30%)、厚壁菌门(Firmicutes)(AG,6.01%;EP,8.24%;SP,3.96%;UV,13.13%)和绿弯菌门(Chloroflexi)(AG,3.98%;EP,8.91%;SP,8.95%;UV,7.59%),主要类群在不同植被带中占比不同;植被带类型对细菌的群落结构特征影响不大,但对细菌种群的多样性影响较大. 典型对应分析(CCA)结果显示,w(TP)、pH和w(OM)(OM为有机质)是影响不同植被带细菌种群结构的主要环境因子.
关键词:  洱海湖滨带  植被带  细菌群落  分布特征  高通量测序
DOI:
分类号:
基金项目:国家水体污染控制与治理科技重大专项(2012ZX07105-002)
Bacteria Distribution Characteristics of Different Vegetation Belts along Shapingwan Riparian Zone of Erhai Lake
ZHAO Xinyan1,2, WANG Haiyan2,3, HOU Zeying2, CHU Zhaosheng2, YANG Yongzhe1
1.School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi′an 710055, China ;2.State Environmental Protection Key Laboratory for Lake Pollution Control, National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, Chinese Research Academy of Environmental Sciences, Beijing 100012, China ;3.Research Center for Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
Abstract:
Abstract: The riparian zone is an important protection barrier for lakes,and the microorganisms in different vegetation belts of the zone play important roles in influent pollutant degradation.To study the bacteria distribution characteristics in different vegetation belts, Shapingwan riparian zone of Erhai Lake was selected as the study area. Illumina HiSeq high throughput sequencing molecular approach was used to study the bacteria diversity and community structure of the arbor grass vegetation belt (AG),emergent plant belt (EP),submerged plant belt (SP) and unvegetated belt (UV).The results showed that the bacteria species were very abundant in different vegetation belts, and there were 54 phyla, 106 classes, 192 orders, 334 families and 503 genera.The vegetation belts with bacteria OTUs number from high to lowwere as follows:EP (3162),AG (2926), UV (2709) and SP(2427), and the vegetation belts with Shannon diversity indices from high to low were EP(10.14),AG (9.75), SP (9.44) and UV (9.26). The predominant bacteria community were Proteobacteria(48.55% for AG, 47.83% for EP, 40.36% for SP and 44.00% for UV),Acidobacteria(19.30% for AG, 5.31% for EP, 5.67% for SP and 6.30% for UV), Firmicutes(6.01% for AG, 8.24% for EP, 3.96% for SP and 13.13% for UV) and Chloroflexi(3.98% for AG, 8.91% for EP, 8.95% for SP and 7.59% for UV), whose proportions in different vegetation belts were different. The results showed that the vegetation belt differences had influences on the bacteria diversity and community characteristics. Canonical correlation analysis indicated that TP,pH and OM were the most important environmental factors which affected the bacterial community in Shangpingwan riparian zone of Erhai Lake.
Key words:  riparian zone of Erhai Lake  vegetation belts  bacterial community  distribution characteristics  high throughput sequencing