引用本文:沈亚琴,魏源,陈志鹏,曾清如,侯红,等.锑胁迫下丛枝菌根真菌对玉米生长与锑吸收及抗氧化酶的影响[J].环境科学研究,2017,30(5):712-719.
SHEN Yaqin,WEI Yuan,CHEN Zhipeng,ZENG Qingru,HOU Hong,et al.Effects of Arbuscular Mycorrhizal Fungi on Growth, Antimony Uptake and Antioxidant Enzymes of Maize under Antimony Stress[J].Reserrch of Environmental Science,2017,30(5):712-719.]
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锑胁迫下丛枝菌根真菌对玉米生长与锑吸收及抗氧化酶的影响
沈亚琴1,2, 魏 源2, 陈志鹏1, 曾清如1, 侯 红2
1.湖南农业大学资源环境学院, 湖南 长沙 410128 ;2.中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
摘要:
为了探讨AM(arbuscular mycorrhizal,丛枝菌根)真菌在Sb(锑)胁迫下对农作物生长及吸收Sb的影响,采用盆栽试验研究了在不同Sb添加量〔即w(Sb)分别为0、500、1 000 mg/kg〕下,接种AM真菌对玉米植株生物量以及N、P和Sb的吸收、膜脂过氧化〔MDA(丙二醛)〕和抗氧化酶活性的影响. 结果表明:随着土壤中Sb添加量的增加,玉米植株的生物量、w(TN)和w(TP)均呈显著下降趋势,植株体内的w(Sb)和Sb积累量、MDA含量、CAT(过氧化氢酶)以及POD(过氧化酶)活性均显著上升. 与未接种组相比,接种AM真菌显著促进了玉米植株的生长,提高了玉米植株地上部分的w(TN)和w(TP). 在3个Sb添加量处理下,接种显著增加了玉米植株地下部分的w(Sb)、地上和地下部分的Sb积累量以及地上部分的CAT活性,增幅分别为8.90%~23.30%、18.87%~28.37%、27.68%~78.95%及14.92%~88.52%;同时,接种降低了玉米植株中Sb的转运率、玉米植株地上部分的w(Sb)和MDA含量,在1000 mg/kg Sb添加量下差异达显著水平,三者分别降低了36.35%、22.81%和24.29%. 研究显示,在Sb污染环境下,接种AM真菌能够减轻玉米植株膜脂过氧化程度,在提高玉米植株地下部分w(Sb)的同时,也会降低Sb向地上部分的转运,减轻Sb对玉米的毒害作用.
关键词:  丛枝菌根真菌  Sb污染土壤  玉米  Sb吸收  抗氧化酶
DOI:
分类号:
基金项目:国家自然科学基金项目(41271338,41303066);科技部科研院所技术开发研究专项(2014EG166135)
Effects of Arbuscular Mycorrhizal Fungi on Growth, Antimony Uptake and Antioxidant Enzymes of Maize under Antimony Stress
SHEN Yaqin1,2, WEI Yuan2, CHEN Zhipeng1, ZENG Qingru1, HOU Hong2
1.College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China ;2.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
Abstract:
Abstract: A pot experiment was conducted to investigate the effects of arbuscular mycorrhizal (AM) fungi on the growth and uptake of antimony (Sb) in plants under Sb stress, based on AM fungi on biomass, nutrient uptake, Sb uptake, malondialdhyde (MDA) and antioxidant enzyme activities by maize grown in soils with different Sb levels (0,0 and 1000 mg/kg). The results showed that the biomass and contents of TN and TP in the maize decreased significantly with the increase of Sb concentration in soil. The contents of Sb, MDA, CAT and POD in the plant also decreased with the increase of Sb concentration. Compared with the uninoculated group, the inoculation of AM fungi promoted the growth of maize and significantly increased the contents of TN and TP in aerial parts. Moreover, for three Sb concentration treatments, underground Sb content of maize increased by 8.90%-23.30%, the aboveground and underground Sb accumulation increased by 18.87%-28.37% and 27.68%-78.95%, while CAT activities in the aboveground part of maize increased by 14.92%-88.52%. Meanwhile, at 1000 mg/kg Sb concentration, the transport rate and the contents of Sb and MDA in the aerial part of maize significantly decreased by 36.35%, 22.81% and 24.29%, respectively. Therefore, the inoculation of AM fungi could reduce the level of membrane lipid peroxidation, increase the content of Sb in the underground part of maize and decrease the transport of Sb to the aerial part, thus alleviating the toxicity of Sb to maize.
Key words:  arbuscular mycorrhizal fungi  Sb-contaminated soils  maize  Sb uptake  antioxidant enzyme