引用本文:黄郑郑,曹刚,陈海升,李紫惠,莫测辉,等.异养硝化好氧反硝化菌协同竞争对脱氮特性的影响[J].环境科学研究,2017,30(5):765-774.
HUANG Zhengzheng,CAO Gang,CHEN Haisheng,LI Zihui,MO Cehui,et al.Synergy and Competition for Nitrogen Removal by Heterotrophic Nitrification-Aerobic Denitrification Bacteria[J].Reserrch of Environmental Science,2017,30(5):765-774.]
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异养硝化好氧反硝化菌协同竞争对脱氮特性的影响
黄郑郑1, 曹 刚1,2, 陈海升1, 李紫惠1, 莫测辉1,2
1.暨南大学环境学院, 广东 广州 510630 ;2.广东省污染控制与修复材料工程技术中心, 广东 广州 510630
摘要:
为了探讨在脱氮过程中异养硝化-好氧反硝化菌类之间的协同和竞争作用,以A2/O工艺好氧污泥中筛出的三株异养硝化-好氧反硝化菌——XH02、XH03和FX03为研究对象,经16S rDNA基因序列系统发育分析,鉴定XH02为人苍白杆菌(Ochrobactrum sp.),XH03和FX03为假单胞菌(Pseudomonas sp.). 在此基础上,分别考察了单菌株和复合菌株(XH02+XH03、XH02+FX03、XH03+FX03和XH02+XH03+FX03)在异养硝化和好氧反硝化条件下的脱氮特性. 结果表明:菌株XH02和XH03具有高效脱氮特性,在异养硝化过程中,第24小时对NH4+-N的去除率分别为90.2%和89.5%;在好氧反硝化过程中,二者在第24小时对NO3--N的去除率分别为91.8%和94.0%. 复合菌株XH02+XH03无论在异养硝化还是好氧反硝化过程中,均能相互协同,促进生长,进一步提高了脱氮效率,在第24小时对NH4+-N和NO3--N的去除率分别达到97.1%和96.7%. 在硝化和反硝化过程中,菌株FX03对XH02、XH03均存在着竞争关系,FX03的存在会抑制菌株XH02和XH03的生长,显著降低脱氮效率. 研究显示,异养硝化-好氧反硝化菌XH02和XH03之间的协同作用可以强化废水生物处理,提高脱氮效率.
关键词:  复合菌株  异养硝化-好氧反硝化  脱氮特性  协同竞争  混合培养
DOI:
分类号:
基金项目:国家基金委-广东省政府联合基金重点项目(U1501233);广东省基金研究团队项目(2016A030312009);广东省环境污染控制与修复材料工程技术研究中心建设项目(2015B090903070)
Synergy and Competition for Nitrogen Removal by Heterotrophic Nitrification-Aerobic Denitrification Bacteria
HUANG Zhengzheng1, CAO Gang1,2, CHEN Haisheng1, LI Zihui1, MO Cehui1,2
1.School of Environment, Jinan University, Guangzhou 510630, China ;2.Guangdong Engineering Center for Environment Contamination Control and Restorative Materials, Guangzhou 510630, China
Abstract:
Abstract: To investigate the effects of synergy and competition by heterotrophic nitrification-aerobic denitrification bacteria on nitrogen removal,the XH02, XH03 and FX03 were isolated from aerobic sludge in A2/O process. They were identified as Ochrobactrum sp., Pseudomonas sp. and Pseudomonas sp. on the basis of phylogenetic analysis of 16S rDNA gene sequence, respectively. Further heterotrophic nitrification and aerobic denitrification analysis were revealed for single and compound strains of XH02+XH03, XH02+FX03, XH03+FX03 and XH02+XH03+FX03. The results showed that XH02 and XH03 exhibited highly efficient denitrification capability. The ammonia removal efficiencies for XH02 and XH03 were 90.2% and 89.5% after 24 hours in the process of heterotrophic nitrification, respectively. The nitrate removal efficiencies were 91.8% and 94.0% after 24 hours during the denitrification, respectively. XH02 and XH03 were able to cooperatively promote growth with each other, and improved nitrogen removal efficiency in the process of nitrification and denitrification. The ammonia and nitrate removals for XH02+XH03 reached 97.1% and 96.7% after 24 hours, respectively. The strain FX03 had a competitive relationship with XH02 and XH03, which inhibited their growth and significantly reduced nitrogen removal efficiency through nitrification and denitrification. The results show that XH02+XH03, which has a positive synergy with each other, has a potential application in wastewater treatment for nitrogen removal under aerobic conditions.
Key words:  compound strains  heterotrophic nitrification-aerobic denitrification  denitrification characteristics  synergy and competition  mixed culture