引用本文:王继玥,刘燕,刘勇,石登红,等.基于高通量测序检测Pb污染对三叶草根际 土壤细菌多样性的影响[J].环境科学研究,2018,31(1):102-110.
WANG Jiyue,LIU Yan,LIU Yong,SHI Denghong,et al.Effect of Lead Pollution on Bacterial Diversity in Rhizosphere of Clover based on High-Throughput Sequencing[J].Reserrch of Environmental Science,2018,31(1):102-110.]
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 282次   下载 256 本文二维码信息
码上扫一扫!
分享到: 微信 更多
基于高通量测序检测Pb污染对三叶草根际 土壤细菌多样性的影响
王继玥1, 刘燕1, 刘勇1, 石登红1,2
1. 贵阳学院生物与环境工程学院, 贵州 贵阳 550005;2. 贵州省山地珍稀动物与经济昆虫重点实验室, 贵州 贵阳 550005
摘要:
为了研究Pb污染对根际土壤微生物多样性的影响,揭示根际微生物对Pb胁迫的适应性机制,通过盆栽试验模拟轻度[w(Pb)为300 mg/kg]、中度[w(Pb)为600 mg/kg]和重度[w(Pb)为900 mg/kg]Pb污染土壤环境,利用高通量测序技术和生物信息学分析方法,测定了不同w(Pb)处理下三叶草根际土壤中细菌群落的丰度和组成.结果表明:①共检测到37个门,其中变形菌门为优势菌群,占比为50.7%~53.9%,其次为拟杆菌门、酸杆菌门和疣微菌门.②共检测到623个属,其中鞘氨醇单胞菌属为优势菌属,占比为17.1%~19.4%.③中度Pb污染土壤样本中细菌的多样性最高,重度Pb污染土壤样本中的细菌数量最少,并且其群落组成与其他处理差异最大.研究显示,重度Pb污染会显著抑制三叶草根际土壤样本中细菌的生长,降低土壤样本中细菌总量及其群落的多样性,但中度Pb污染会提高土壤样本中细菌群落的多样性.Pb污染会改变三叶草根际土壤样本中细菌群落组成和丰度,不同类型细菌对Pb污染土壤的适应性不同.根际土壤样本中Gp1菌群的丰度随着土壤w(Pb)的增加而增加,说明Gp1菌群可能是具有Pb污染抗性的优势菌群.
关键词:  Pb  根际土壤  生物多样性  细菌群落
DOI:10.13198/j.issn.1001-6929.2017.03.60
分类号:X5
基金项目:贵州省科学技术基金项目(黔科合J字LKG[2013]23号);有机蔬菜生态栽培繁育产学研合作基地项目(黔教合KY字[2013]125)
Effect of Lead Pollution on Bacterial Diversity in Rhizosphere of Clover based on High-Throughput Sequencing
WANG Jiyue1, LIU Yan1, LIU Yong1, SHI Denghong1,2
1. School of Biological and Environmental Engineering, Guiyang College, Guiyang 550005, China;2. Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Guiyang 550005, China
Abstract:
This paper is intended to study the effect of Pb pollution on microbial diversity in the rhizosphere soil, and the adaptive mechanism of rhizosphere microorganisms to lead (Pb) stress. A pot experiment of white clover (Trifolium repens L.) was conducted in simulated slight (w(Pb)=300 mg/kg), moderate (w(Pb)=600 mg/kg), and severe(w(Pb)=900 mg/kg) Pb polluted soil environments. Test soil and clover seedlings were collected from a vegetable field of the experimental farm without Pb pollution in Guiyang College. Community abundance and composition of the rhizosphere bacteria of clover plants under different soil Pb treatments were detected by the high-throughput sequencing detection technique and the bioinformatics method. Results showed that 37 phyla were detected, in which proteobacteria was the dominant flora with a proportion from 50.7% to 53.9%, and followed by bacteroides, acid bacillus, and verrucomicrobia. In the genus taxa, 623 genera ware identified, in which sphingomonas was the dominant genus with a proportion from 17.1% to 19.4%. The bacteria diversity was highest in the moderately polluted soil, and lowest in the severe polluted soil, and the composition of bacteria community was obviously different from the other treatments. It was suggested that severe Pb pollution significantly inhibited the growth and reproduction of the rhizosphere bacteria of white clover, and reduced their population and community diversity. However, population diversity of the rhizosphere bacteria was increased under the moderate soil pollution condition. Soil Pb pollution might change composition and abundance of the rhizosphere bacteria of white clover, and there was different in adaptability on the pollution conditions among types of the rhizosphere bacteria. The abundance of genus Gp1 bacteria in the rhizosphere soil increased with the increase of Pb concentrations of the soil. It suggested that genus Gp1 might be the dominant flora with lead pollution resistance.
Key words:  Pb  rhizosphere soil  biodiversity  bacterial community