引用本文:贺婧,闫冰,李俊生,等.秦岭中段北坡不同海拔土壤中细菌群落的分布特征及区域差异比较[J].环境科学研究,2019,32(8):1374-1383.
HE Jing,YAN Bing,LI Junsheng,et al.Altitude Distribution Patterns and Regional Differences of Soil Bacterial Community in Northern Slopes in the Middle Qinling Mountains[J].Research of Environmental Sciences,2019,32(8):1374-1383.]
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秦岭中段北坡不同海拔土壤中细菌群落的分布特征及区域差异比较
贺婧1,2, 闫冰1,3, 李俊生1, 付刚1, 齐月1, 马艺文1,2, 乔梦萍1,2
1. 中国环境科学研究院, 国家环境保护区域生态过程与功能评估重点实验室, 北京 100012;2. 中国人民大学环境学院, 北京 100872;3. 江西省科学院能源研究所, 江西 南昌 330096
摘要:
为深入理解土壤细菌群落沿海拔梯度的分布特征,以秦岭山脉中段的朱雀国家森林公园朱雀山北坡(简称“朱雀北坡”)与太白山自然保护区太白山北坡(简称“太白山北坡”)为研究区域,海拔梯度上每隔400 m设置采样点,通过高通量测序研究土壤细菌群落的海拔变化规律及其关键影响因素.结果表明:研究区域内,随海拔升高,土壤pH趋于降低(太白山北坡不明显),w(TC)、w(TN)、w(TOC)均显著升高,w(TP)也趋于升高(朱雀北坡不明显).两个区域土壤细菌中相对丰度最高的均为变形菌门和酸杆菌门,朱雀北坡随海拔梯度变化显著的细菌主要包括变形菌门、硝化螺旋菌门、浮霉状菌门、酸杆菌门、放线菌门,其中,硝化螺旋菌门相对丰度与海拔呈正相关,放线菌门相对丰度与海拔呈负相关;太白山北坡随海拔梯度变化显著的细菌主要包括放线菌门、疣微菌门、浮霉状菌门、绿弯菌门,其中,放线菌门相对丰度与海拔呈负相关,其余三者相对丰度与海拔呈正相关.两个区域土壤细菌多样性指数(如Shannon-Wiener指数、Chao1指数、observed-species指数、PD_whole_tree指数等)沿海拔梯度均无明显变化规律,但太白山北坡数值整体上高于朱雀北坡.典范对应分析(canonical correlation analysis,CCA)结果显示,影响朱雀北坡和太白山北坡土壤细菌群落结构的主要因素均为pH和w(TOC).研究显示,不同海拔梯度也是导致秦岭中段北坡土壤细菌群落差异的重要因素.
关键词:  秦岭北坡  细菌多样性  海拔梯度  空间异质性
DOI:10.13198/j.issn.1001-6929.2019.01.12
分类号:X172
基金项目:国家重点研发计划项目(No.2016YFC1201100);环境保护部生物多样性调查与评估专项(No.2096001006);江西省科学院重点科研项目(No.2018-YZD2-07)
Altitude Distribution Patterns and Regional Differences of Soil Bacterial Community in Northern Slopes in the Middle Qinling Mountains
HE Jing1,2, YAN Bing1,3, LI Junsheng1, FU Gang1, QI Yue1, MA Yiwen1,2, QIAO Mengping1,2
1. State Environmental Protection Key Laboratory of Regional Eco-Process and Function Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;2. School of Environment and Nature Resources, Renmin University of China, Beijing 100872, China;3. Institute of Energy, Jiangxi Academy of Science, Nanchang 330096, China
Abstract:
To understand the distribution of soil microbial community across elevation gradient, a study was performed in the northern slope of Zhuque National Forest Park (the northern slope of Zhuque) and the northern slope of Taibai Mountain Nature Reserve (the northern slope of Taibai Mountain) in the middle of the Qinling Mountains. A gradient was set every 400 m to study the altitude variation of soil bacterial diversity and its key influencing factors through high-throughput sequencing. The results showed that with the increase of altitude, the soil pH tended to decrease (the northern slope of the Taibai Mountain was not obvious), soil w(TC), w(TN) and w(TOC) increased significantly, and soil w(TP) tended to raise (the northern slope of Zhuque was not obvious). The dominant phyla (highest relative abundance) of soil bacteria in the two study areas were Proteobacteria and Acidobacteria. The main bacterial phyla in the northern slope of Zhuque changed significantly along the elevation gradient, including Proteobacteria, Nitrospirae (positive correlation with elevation gradient) and Firmicutes, Acidobacteria, and Actinobacteria (negative correlation with elevation gradient). The main bacterial phyla in northern slope of Taibai Mountain changed significantly along the elevation gradient, including Actinobacteria (negative correlation with elevation gradient) and Verrucomicrobia, Planctomycete, and Chloroflex (positive correlation with elevation gradient). Soil bacterial diversity indexes such as Shannon-Wiener, Chao1, observed-species and PD_whole_tree showed no significant change along the elevation gradient in the two study areas, but these indexes were higher in northern slope of the Taibai Mountain compared to the northern slope of Zhuque. Canonical correlation analysis (CCA) showed that pH and w(TOC) were the main factors affecting the soil bacterial community structure on the northern slope of Zhuque and the northern slope of Taibai Mountain. The elevation gradient had a strong influence on soil bacterial community in the northern slope of the middle Qinling Mountains.
Key words:  northern slope of the Qinling Mountains  bacterial diversity  elevation gradient  spatial heterogeneity