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不同催花肥对莲雾根际土壤细菌群落多样性的影响
吴斌, 刘永霞, 王必尊, 何应对, 丁哲利, 王丽霞, 王安邦
中国热带农业科学院海口实验站
摘要:
为了解在反季节催花期间不同催花肥处理下莲雾根际土壤细菌群落结构的影响,采用Illumina MiSeq高通量测序技术对细菌16S rRNA V3-V4区进行检测,并结合土壤理化性质,比较分析不施肥(CK)与两种不同浓度施肥处理组(IF组,即无机肥处理组;GM组,即羊粪有机肥处理组)下的根际土壤细菌群落结构多样性. 结果表明:①所有样品中共检测到10 925个OTU(Operational Taxonomic Unit),包括了239 639条有效序列,可分为28个门,411个属. ②多样性指数分析显示,细菌群落多样性顺序为IFM (中浓度无机肥处理)>GML(低浓度羊粪有机肥处理)>IFL(低浓度无机肥处理)>GMM(中浓度羊粪有机肥处理)>CK(对照)>IFH(高浓度无机肥处理)>GMH(高浓度羊粪有机肥处理). 其中,酸杆菌门(Acidobacteria) 、绿弯菌门(Chloroflexi)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)为主要菌群,所占比例超过总数的67.33%. ③环境因子的相关性热图分析表明,装甲菌门(Armatimonadetes)、硝化螺旋菌门(Nitrospirae) 、酸杆菌门、放线菌门、厚壁菌门(Firmicutes)这五类菌群与不同环境因子的显著性关系如下:装甲菌门与pH呈现出极显著正相关,硝化螺旋菌门分别与全氮(TN)和有机质(OM)呈现出显著负相关,酸杆菌门与速效磷(P)呈现出显著负相关,放线菌门与速效磷(P)呈现出显著正相关,厚壁菌门分别与速效磷(P)和速效钾(K)呈现出极显著正相关. ④不同施肥条件下莲雾根际土壤细菌群落结构特征分析显示,CK、IF组和GM组所特有的OTU数分别占总比例的0.51%、7.08%和2.60%,表明不同肥料的添加对土壤细菌群落多样性产生了一定的影响,在IFH和GMH的分别处理下,酸杆菌门的Subgroup_2_norank属与绿弯菌门的JG37-AG-4_norank属的相对丰度最高,分别为13.66%和16.42%. 本研究结果表明,适量施加无机肥或羊粪有机肥,可以显著提高土壤细菌的丰富度和多样性,有利于土壤生态环境的改良与维系.
关键词:  莲雾  羊粪有机肥  无机肥  细菌群落  Illumina MiSeq高通量测序
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Effects of Different Flower Forcing Fertilizers on Bacterial Community Diversity in Rhizosphere Soil of Wax Apple
wubin, liuyongxia, wangbizun, heyingdui, dingzheli, wanglixia, wanganbang
Haikou Experimental Station, Chinese Academy of Tropical Agricultural
Abstract:
In order to understand the effect of different treatments of flower fertilizers on microbial community structure in rhizosphere soil of Wax Apple during the anti-season flower forcing period, the bacterial 16S rRNA V3-V4 region was detected by the Illumina MiSeq high-throughput sequencing technology. And combined with physical and chemical properties of soil, microbial community structure diversity in rhizosphere soil of no fertilization group (CK) and two different concentrations of fertilization treatments group (IF group, ie, inorganic fertilizer treatment group, GM group, ie sheep manure organic fertilizer treatment group) was analyzed. The results showed that (1) 10,925 OTU (Operational Taxonomic Unit) were detected in all samples, including 239,639 valid sequences, which could be divided into 28 phylum and 411 genus. (2) The diversity index analysis showed that the order of bacterial community diversity was: IFM (inorganic fertilizer treatment of medium concentration)> GML (sheep manure organic fertilizer treatment of low concentration)> IFL (inorganic fertilizer treatment of low concentration)> GMM (sheep manure organic fertilizer treatment of medium concentration) > CK (control group)> IFH (inorganic fertilizer of high concentration) > GMH (sheep manure organic fertilizer treatment of high concentration) . And Acidobacteria, Chloroflexi, Proteobacteria and Actinobacteria were main microflora accounting for 67.33% of the total. (3) Correlation thermal imagine analysis for environmental factors showed that the relationship between the five types of microflora (Armatimonadetes, Nitrospirae, Acidobacteria, Actinobacteria and Firmicutes) and the environmental factors is as follows, the Armatimonadetes has showed a very significant positive correlation with pH, the Nitrospirae has showed a significant negative correlation with TN and OM respectively, the Acidobacteria has showed a significant negative correlation with available P, the Actinobacteria has showed a significant positive correlation with available P, the Firmicutes has showed a very significant negative correlation with available P and available K respectively. (4) The characteristic analysis of bacterial community structure of Rhizosphere Soil under different fertilization conditions showed that the specific number of OTU in CK group, IF group and GM group accounted for 0.51%, 7.08% and 2.60% of the total, respectively, indicating that the different fertilizers treatment had a certain effect on the diversity of soil bacterial communities. Under the treatment of IFH and GMH, the relative abundance of Subgroup_2_norank of Acidobacteria and the JG37-AG-4_norank of Chloroflexi was highest, which was 13.66% and 16.42%, respectively. The study showed that the proper treatment of inorganic fertilizer or sheep manure organic fertilizer could significantly increase the abundance and diversity of soil bacteria, which was beneficial for the improvement and maintenance of soil ecological environment.
Key words:  Wax Apple  goat manure organic fertilizer  inorganic fertilizer  bacterial communities  Illumina MiSeq high-throughput sequencing