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采用高效纤维质降解菌群处理木薯酒精废液及其厌氧发酵动力学研究
卞志明,赵明星,廖家林,阮文权,等
作者单位E-mail
卞志明 江南大学环境与土木工程学院 bzmingjn@163.com 
赵明星 江南大学环境与土木工程学院  
廖家林 江南大学环境与土木工程学院  
阮文权 江南大学环境与土木工程学院 wqruan@jiangnan.edu.cn 
摘要:
为提高木薯酒精废液中固形物的降解效率,优化木薯酒精废液的厌氧发酵特性,本文采用高效纤维质降解复配菌种作为CSTR接种污泥,在高温条件下,通过逐级提高进料负荷,研究不同容积负荷下的厌氧消化性能及相应的酶活性变化,建立了厌氧发酵过程动力学模型.研究结果表明,经过长时间稳定运行,在容积负荷为14kgCOD/m3.d条件下,反应器出水TCOD和SCOD分别为15367.6、10982.8mg/L,TCOD去除率达到70-75%,甲烷含量在48%左右,沼气产率为0.22L/gTCOD;此外,木聚糖酶活性、纤维素酶活性在该条件下达到最大值,分别为42.1U、30.2U,脱氢酶活性在容积负荷为12kgCOD/m3.d时达到最大值80.1TFμg/h.mL.动力学模型研究表明,通过拟合直线可以得到最大原料产气率ym为0.335L/gTCOD,以及一级反应常数k为0.6363d-1,在预期产气率为y(t)值0.3 L/gTCOD,通过该模型可以获得该条件下最佳水力停留时间 ( HRT )值13.5d和容积负荷(Fr)值4.5 kgCOD/m3.d.
关键词:  厌氧发酵  高温  木薯酒精废液  酶活  动力学模型
DOI:
分类号:
基金项目:国家自然科学基金资助(51678279);国家科技支撑计划课题资金资助(2014BAC25B01)
Study on the treatment of cassava alcohol wastewater by using efficient cellulose degrading microbial and the anaerobic fermentation kinetics
BIAN Zhiming,ZHAO Mingxing,LIAO Jialin,RUAN Wenquan,et al
Abstract:
In order to improve the solid organics degradation rate of cassava alcohol wastewater and optimize its anaerobic fermentation characteristics, the high-efficiency cellulose degradation microbial was used as inoculum in this CSTR experiment. The main research was to investigate the anaerobic fermentation characteristics, enzyme activity changes as well as the kinetic model of anaerobic digestion by the increasing of volume loading rate under the thermophilic conditions. The results showed that the effluent concentration of TCOD and SCOD were 15367.6, 10982.8mg/L ,respectively, and the total removal rate of TCOD was 70-75%, methane content maintained at about 48%, the biogas generation rate was 0.22L/gTCOD when the volume loading rate was 14 kgCOD/m3.d. Besides, xylanase activity and cellulose enzyme activity reached its maximum value under this condition, which were 42.1U, 30.2U, respectively. In addition, the dehydrogenase activity reached the maximum value of 80.1TFμg/h.mL when the volume loading rate was 12 kgCOD/m3.d.The kinetic model results indicated that the maximum raw material biogas production rate ym was 0.335L/gTCOD and the first order reaction constant k was 0.6363d-1 according to the fitting curve, it could figure out the optimized hydraulic retention time (HRT) of 13.5 and the volume loading rate(Fr) of 4.5 kgCOD/m3.d by the given anaerobic fermentation rate of y(t) 0.3 L/gTCOD.
Key words:  Anaerobic fermentation  Thermophilic temperature  Enzyme activity  Kinetic model