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基于高通量测序检测铅污染对三叶草根际土壤细菌多样性的影响
王继玥
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王继玥 贵阳学院 acute2803764@163.com 
摘要:
为了研究铅污染对根际土壤微生物多样性的影响,揭示根际微生物对铅胁迫的适应性机制,通过盆栽实验模拟轻度(300 mg/kg)、中度(600 mg/kg)和重度(900 mg/kg)铅(Pb)污染土壤环境。供试土壤采自贵阳学院试验田无Pb污染菜地,供试白三叶草(Trifolium repens Linn)幼苗采自同一菜地区。利用高通量测序技术和生物信息学分析方法,测定了不同浓度铅处理下三叶草根际土壤中细菌群落的丰度和组成。结果表明:在门水平共检测到37个门,其中变形菌门为优势菌群,所占比例为50.7%~53.9%,其次为拟杆菌门、酸杆菌门和疣微菌门。在属分类上共检测到623个属,其中鞘氨醇单胞菌属为优势菌属,所占比例在17.1%~19.4%之间。中度污染w(Pd)(600 mg/kg)土壤样本中细菌的多样性最高,重度污染w(Pd)(900mg/kg)土壤中的细菌数量最少,且其群落组成与其他处理差异最大。说明重度铅污染会显著抑制三叶草根际土壤细菌的生长,降低土壤中细菌总量及其群落的多样性,但中度铅污染会提高土壤细菌群落的多样性。铅污染会改变三叶草根际土壤细菌群落组成和丰度,不同类型细菌对铅污染土壤的适应性不同。根际土壤样本中菌Gp1属的丰度随着土壤铅浓度的增加而增加,说明Gp1可能是具有铅污染抗性的优势菌群。
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The Effect of Lead Pollution on Bacterial Diversity in Rhizosphere of Clover Based on High-throughput Sequencing
WANG JIYUE
Abstract:
This paper is intended to study the effect of Pb pollution on microbial diversity in the rhizosphere soil, and the adaptive mechanism of rhizosphere microorganisms to lead (Pb) stress. A pot experiment of white clover (Trifolium repens Linn) was conducted in simulated slight (300 mg/kg), moderate (600 mg/kg), and severe (900 mg/kg) Pb polluted soil environments. Test soil and clover seedlings were collected from a vegetable field of the experimental farm in Guiyang College, without Pb pollution. Community abundance and composition of the rhizosphere bacteria of clover plants under different soil Pb treatments were detected by the high-throughput sequencing detection technique and the bioinformatics method. Results showed that in the phyla classification, 37 of phyla were detected out, of which proteobacteria was the dominant flora with a proportion from 50.7% to 53.9%, and followed by bacteroides, acid bacillus, and verrucomicrobia. In the genus classifications, 623 of the genus ware detected out, of which sphingomonas was the dominant genus with a proportion from 17.1% to 19.4%. The bacteria diversity was highest in the moderately polluted soil, and lowest in the severe polluted soil, and the composition of bacteria community was obviously different from the other treatments. It was suggested that severe Pb pollution significantly inhibited the growth and reproduction of the rhizosphere bacteria of white clover, and reduced their population and community diversity. However, population diversity of the rhizosphere bacteria was increased in the moderate soil pollution condition. Soil Pb pollution might change composition and abundance of the rhizosphere bacteria of white clover, and there was different in adaptability on the pollution conditions among types of the rhizosphere bacteria. The abundance of genus Gp1 bacteria in the rhizosphere soil increased with the increase of Pb concentrations of the soil. It suggested that genus Gp1 might be the dominant flora with lead pollution resistance.
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