富油微藻-真菌共培养资源化处理畜禽养殖废水的效能研究
Performance of lipid-rich microalgal -fungal co-culture for the treatment of simulated livestock wastewater
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摘要: 构建富油小球藻(Chlorella vulgaris)和米曲霉菌(Aspergillus oryzae)的共培养体系,探究了不同初始藻菌接种比例对模拟畜禽养殖废水中污染物去除及体系中生物质变化的影响.研究结果表明:C. vulgaris和A. oryzae在废水处理过程中具有协同效应,与C. vulgaris单独处理相比,共培养更有利于污染物的去除.最优藻菌接种比例为25:1,总氮(TN)、氨氮(NH4%2B-N)、总磷(TP)、化学需氧量(COD)和铜离子(Cu(II))的去除率分别达72.51%、71.19%、92.23%、91.47%和90.38%.共培养体系对3种磺胺类药物(SAs)也具有显著的生物去除效果,磺胺二甲嘧啶(SMZ)、磺胺甲恶唑(SMX)和磺胺间甲氧嘧啶(SMM)的去除率分别达57.61%、58.31%和50.48%,与微藻单独处理相比分别提高了34.89%、25.43%和45.71%.丝状真菌易于与微藻形成球状颗粒物,从而实现对微藻的有效采收,最大采收效率为76.91%,采收后的生物量达1.26 g/L.藻菌生物质中蛋白质、多糖和油脂的含量分别为111、438和249 mg/g.因此,C. vulgaris和A. oryzae共培养不仅有效去除了废水中的各类污染物,而且实现了生物质的资源化利用,在畜禽养殖废水处理中具有潜在的工程应用价值.Abstract: A co-culture system of lipid-rich Chlorella vulgaris and Aspergillus oryzae was established to investigate the effects of different initial microalgal and fungal inoculation ratios on pollutant removals and biomass change in simulated livestock wastewater. The results showed that C. vulgaris and A. oryzae had synergistic effects in wastewater treatment, and co-culture system was more beneficial to the removal of pollutants than C. vulgaris alone. The optimal inoculation ratio of C. vulgaris-A. oryzae was 25:1, and the removal efficiencies of total nitrogen (TN), ammonia nitrogen (NH4%2B-N), total phosphorus (TP), chemical oxygen demand (COD) and copper were 72.51%, 71.19%, 92.23%, 91.47% and 90.38%, respectively. In addition, the co-culture system also showed good biological removal for three sulfonamides. The removals of sulfadiazine (SMZ), sulfamethoxazole (SMX) and sulfamethoxine (SMM) were 57.61%, 58.31% and 50.48%, respectively, which were 34.89%, 25.43% and 45.71% higher than those of microalgae alone. Filamentous fungi were easy to form spherical particles with microalgae to realize the harvesting of microalgae. The maximum harvesting efficiency was 76.91%, and the biomass after harvesting reached 1.26 g/L. The contents of protein, polysaccharide and lipid were 111, 438 and 249 mg/g, respectively. Therefore, the co-culture of C. vulgaris and A. oryzae not only effectively removed the pollutants in livestock wastewater, but also realized the resource utilization of biomass, which exhibits potential application in livestock wastewater treatment.
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Key words:
- microalgal-fungal co-culture /
- livestock wastewater /
- sulfonamides /
- biodiesel production
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