铜绿微囊藻高温胁迫后的生长补偿效应

李 婷; 景元书; 韩 玮; 罗菊花

铜绿微囊藻高温胁迫后的生长补偿效应

1.
南京信息工程大学应用气象学院, 气象灾害预报预警与评估协同创新中心, 江苏南京 210044 ;河北省气象局, 河北石家庄 050022
2.
南京信息工程大学应用气象学院, 气象灾害预报预警与评估协同创新中心, 江苏南京 210044 ;南京信息工程大学应用气象学院, 江苏省农业气象重点实验室, 江苏南京 210044
3.
中国科学院南京地理与湖泊研究所, 江苏南京 210008

基金项目: 江苏省科技支撑计划项目(BE2011840)；江苏省农业气象重点实验室开放基金项目(JKLAM201205)；江苏高校优势学科建设工程项目(PAPD002)

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出版历程
- 刊出日期: 2015-04-25

Compensatory Growth Effect of Microcystis aeruginosa after High Temperature Stress

1.
Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, School of Applied Meteorology of Nanjing University of Information Science and Technology, Nanjing 210044, China ;Hebei Meteorological Bureau, Shijiazhuang 050022, China
2.
Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, School of Applied Meteorology of Nanjing University of Information Science and Technology, Nanjing 210044, China ;Jiangsu Key Laboratory of Agricultural Meteorology, School of Applied Meteorology of Nanjing University of Information Science and Technology, Nanjing 210044, China
3.
Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China

摘要

摘要: 为探究藻类受高温胁迫后的恢复生长情况,以铜绿微囊藻(Microcystis aeruginosa)为材料,设置了40 ℃高温组及25 ℃对照组,分别处理3、6和12 d后转入25 ℃进行恢复培养30 d,测定生长过程中的细胞密度以及Chla(叶绿素a)、CAR(类胡萝卜素)、Pro(可溶性蛋白)、Sug(可溶性糖)和丙二醛的含量. 结果表明:40 ℃高温下铜绿微囊藻的生长受到显著抑制,胁迫至第12天时,细胞密度、ρ(Chla)和ρ(CAR)分别比对照组降低了81.08%、97.89%和90.31%. 解除胁迫后,40 ℃处理3 d组呈现一定的超补偿,细胞密度、ρ(Chla)和ρ(CAR)在恢复培养30 d的平均补偿指数分别为1.63、0.90和2.12；40 ℃处理6和12 d组呈现低补偿,恢复培养至第30天时,ρ(Pro)分别比对照组降低了14.86%和48.75%,ρ(Sug)分别比对照组降低了18.77%和53.73%. 胁迫时间越长,丙二醛含量越高,长时间(12 d)的高温胁迫会对细胞造成永久伤害. 采用Logistic方程拟合生长曲线,40 ℃处理6 d组在恢复培养24 d后出现明显的超补偿,细胞密度增大,预计39 d后超过40 ℃处理3 d组. 高温诱发超补偿生长与自然界中藻类暴发的过程具有密切关系,这种内源性因素及藻体生理特征的变化可为湖泊水华发生的理论研究提供依据.
- 高温胁迫 /
- 铜绿微囊藻 /
- 补偿指数 /
- 丙二醛 /
- 超补偿
Abstract: Abstract:Cyanobacteria blooms have become a global problem that destroy water environments. Frequent algae blooms are influenced by short-term changes in temperature. Some studies have shown that several microalgae, such as Pavlova viridis and Tetraselmis tetrethele, apparently show over-compensatory growth after UV-B or nutrient deficiency stress, but there is little information about the over-compensatory growth of microalgae after high temperature stress. To study the recovery growth of algae after high temperature stress, Microcystis aeruginosa was used as an experimental material. Two experiments were conducted. Three groups of M. aeruginosa were cultured under 40 ℃ respectively for 3,6, and 12 days before being transferred to 25 ℃ conditions with the same inoculation density as in the first experiments. The second experiment was set as the control, in which M. aeruginosa was cultured under 25 ℃ the entire time. Cell density, chlorophyll-a, carotenoid, incellular protein, carbohydrate and malondialdehyde were measured during the process. The results showed that under the high temperature stress, the growth of M. aeruginosa was inhibited significantly, with decreases of 81.08%, 97.89% and 90.31% in cell density, chlorophyll-a and carotenoid, respectively, on the 12^th day. After the relief of the high temperature stress, the 40℃ for three days group showed over-compensation, with 30-day average compensation indexes of 1.63,0.90 and 2.12 for cell density, chlorophyll-a and carotenoid, respectively. The two groups of 40 ℃ for 6 and 12 days showed under-compensation. On the 30^th day, the protein contents of the two groups were 14.86% and 48.75%, and the carbohydrate contents were 18.77% and 53.73% lower than the control, respectively. The longer the period of stress, the higher the content of malondialdehyde, and long period stress (12 days) could cause irreversible harm to algae cells. The growth curves fitted with Logistic model indicated that the 40 ℃ for 6 days group would show obvious over-compensation after 24 days recovery, and have a larger cell density than the 40 ℃ for 3 days group would have after the 39^th day recovery. The over-compensation effect induced by high temperature stress was closely related to algal blooming processes. The endogenous factor and the change of algae physiological characteristics may provide basic support for lake algae bloom theoretical research.
- high temperature stress /
- Microcystis aeruginosa /
- compensation index /
- malondialdehyde /
- over-compensation

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参考文献(35)

[1]	PAERL H W,OTTEN T G.Harmful cyanobacterial blooms:causes,consequences,and controls.Microbial Ecology,2013,5:995-1010.
[2]	谢晓玲,周蓉,邓自发.光、温限制后铜绿微囊藻和斜生栅藻的超补偿生长与竞争效应.生态学报,2014,4(5):1224-1234.XIE Xiaoling,ZHOU Rong,DENG Zifa.Overcompensation and competitive effects of Microcystis aeruginosa and Scenedesmus obliquus after low temperature and light stresses.Acta Ecologica Sinica,2014,4(5):1224-1234.
[3]	HUBER V,WAGNER C,GERTEN D,et al.To bloom or not to bloom:contrasting responses of cyanobacteria to recent heat waves explained by critical thresholds of abiotic drivers.Oecologia,2012,9:245-256.
[4]	LIU Xia,LU Xiaohua,CHEN Yuwei.The effects of temperature and nutrient ratios on Microcystis blooms in Lake Taihu,China:an 11-year investigation.Harmful Algae,2011,0(3):337-343.
[5]	QIN Boqiang,ZHU Guangwei,GAO Guang,et al.A drinking water crisis in Lake Taihu,China:linkage to climatic variability and lake management.Environmental Management,2010,5(1):105-112.
[6]	金相灿,储昭升,杨波,等.温度对水华微囊藻及孟氏浮游蓝丝藻生长、光合作用及浮力变化的影响.环境科学学报,2008,8(1):50-55.JIN Xiangcan,CHU Zhaosheng,YANG Bo,et al.Effects of temperature on growth,photosynthesis and buoyancy regulation of the cyanobacteria Microcystis flos-aquae and Planktothrix mougeotii.Acta Scientiae Circumstantiae,2008,8(1):50-55.
[7]	段舜山,郭羽丰,刘振乾,等.四列藻在营养限制胁迫下的超补偿生长研究.生态学报,2003,3(7):1297-1304.DUAN Shunshan,GUO Yufeng,LIU Zhenqian,et al.Over-compensatory growth of Tetraselmis tetrethele under the stress of nutrients deficiency.Acta Ecologica Sinica,2003,3(7):1297-1304.
[8]	BELSKY A J.Does herbivory benefit plants? a review of the evidence.American Naturalist,1986,7(6):870-892.
[9]	刘晓娟,段舜山,李爱芬.绿色巴夫藻受紫外(UV-B)胁迫后的超补偿生长效应.应用生态学报,2007,8(1):169-173.LIU Xiaojuan,DUAN Shunshan,LI Aifen.Overcompensation effect of Pavlova viridis under ultraviolet (UV-B) stress.Chinese Journal of Applied Ecology,2007,8(1):169-173.
[10]	郭羽丰,段舜山,李爱芬,等.四列藻在光限制胁迫下的超补偿生长响应.生态科学,2004,3(1):5-8.GUO Yufeng,DUAN Shunshan,LI Aifen.Over-compensatory growth of Tetraselmis tetrethele in response to the stress of light limitation.Ecologic Science,2004,3(1):5-8.
[11]	STANIER R Y,KUNIASWA R,MANDEL M,et al.Purification and properties of unicellular blue-green algae (Order Chrococcales).Bacteriological Reviews,1971,5:171-205.
[12]	郭羽丰,段舜山,陈洁,等.绿色巴夫藻和四列藻种间竞争机制研究.生态学杂志,2002,1(6):11-14.GUO Yufeng,DUAN Shunshan,CHEN Jie,et al.Mechanism of interspecific competition between Pavlova viridis and Tetraselmis tetrethele.Chinese Journal of Ecology,2002,1(6):11-14.
[13]	郭美婷,胡洪营,陈健,等.紫外线对铜绿微囊藻的抑制效果及特性研究.环境科学,2011,2(6):1608-1613.GUO Meiting,HU Hongying,CHEN Jian,et al.Inhibitory effects of ultraviolet irradiation on the growth of Microcystis aeruginosa.Environmental Science,2011,2(6):1608-1613.
[14]	国家环境保护总局.水和废水监测分析方法.4版.北京:中国环境科学出版社,2002:670-671.
[15]	陈建勋,王晓峰.植物生理学试验指导.广州:华南理工大学出版社,2006:24-25.
[16]	李合生.植物生理生化试验原理和技术.北京:高等教育出版社,2000:199-200.
[17]	李磊,侯文华.荷花和睡莲种植水对铜绿微囊藻生长的抑制作用研究.环境科学,2007,8(10):2180-2186.LI Lei,HOU Wenhua.Inhibitory effects of liquor cultured with Nelumbo nucifera and Nymphaea tetragona on the growth of Microcystis aeruginosa.Environmental Science,2007,8(10):2180-2186.
[18]	王海洋,杜国祯,任金吉.种群密度与施肥对垂穗披碱草刈割后补偿作用的影响.植物生态学报,2003,7(4):477-483.WANG Haiyang,DU Guozhen,REN Jinji.The impacts of population density and fertilization on compensatory responses of Elymus nutans to mowing.Acta Phytoecologica Sinica,2003,7(4):477-483.
[19]	MASCHINSKI J,WHITHAM T G.The continuum of plant responses to herbivory:the influence of plant association,nutrient availability and timing.American Naturalist,1989,4:1-19.
[20]	汤俊,宋立荣,孙松松,等.低光低温联合作用对铜绿微囊藻复苏能力的影响.环境科学,2010,1(12):2932-2937.TANG Jun,SONG Lirong,SUN Songsong,et al.Recruitment ability of Microcystis aeruginosa under low light-low temperature combination.Environmental Science,2010,1(12):2932-2937.
[21]	王健,周天艺,高文程,等.低氧化还原电位对铜绿微囊藻(Microcystis aeruginosa)生长的抑制及恢复的影响.湖泊科学,2012,4(4):528-534.WANG Jian,ZHOU Tianyi,GAO Wencheng,et al.Growth inhibition and recovery from low oxidation reduction potential to Microcystis aeruginosa.Journal of Lake Sciences,2012,4(4):528-534.
[22]	LORETI E,BELLIS L D,ALPI A,et al.Why and how do plant cells sense sugars?.Annals of Botany,2001,8(5):803-812.
[23]	周智彬,徐新文,杨兰英.三种固沙植物对高温胁迫的生理响应及其抗热性研究.干旱区地理,2005,8(6):824-830.ZHOU Zhibin,XU Xinwen,YANG Lanying.Physiological response and high-temperature resistance of three species of psammophytes under high-temperature stress.Arid Land Geography,2005,8(6):824-830.
[24]	陈家长,孟顺龙,胡庚东,等.温度对两种蓝藻种间竞争的影响.生态学杂志,2010,9(3):454-459.CHEN Jiachang,MENG Shunlong,HU Gengdong,et al.Effects of temperature on interspecific competition between two blue-green algae.Chinese Journal of Ecology,2010,9(3):454-459.
[25]	茅华,许海,刘兆普,等.不同起始细胞数量对旋链角毛藻和中肋骨条藻种群竞争的影响.海洋环境科学,2008,7(5):458-461.MAO Hua,XU Hai,LIU Zhaopu,et al.Effect of initial cell density on population competition between Skeletonema costatum and Chaetoceros curvisetus.Marine Environmental Science,2008,7(5):458-461.
[26]	ZHANG Min,DUAN Hongtao,SHI Xiaoli,et al.Contributions of meteorology to the phenology of cyanobacterial blooms:implications for future climate change.Water Research,2012,6:442-452.
[27]	吴攀,邓建明,秦伯强,等.水温和营养盐增加对太湖冬、春季节藻类生长的影响.环境科学研究,2013,6(10):1064-1071.WU Pan,DENG Jianming,QIN Boqiang,et al.Effects of enhanced water temperature and nutrient concentration on algal growth in winter and spring season in Lake Taihu,China.Research of Environmental Sciences,2013,6(10):1064-1071.
[28]	CHU Zhaosheng,JIN Xiangcan,IWAMI N,et al.The effect of temperature on growth characteristics and competitions of Microcystis aeruginosa and Oscillatoria mougeotii in a shallow,eutrophic lake simulator system.Hydrobiologia,2007,1(1):217-223
[29]	YANG Zhou,GENG Linlin,WANG Wei,et al.Combined effects of temperature,light intensity,and nitrogen concentration on the growth and polysaccharide content of Microcystis aeruginosa in batch culture.Biochemical Systematics and Ecology,2012,1:130-135.
[30]	XU Zhenzhu,ZHOU Guangsheng.Combined effects of water stress and high temperature on photosynthesis,nitrogen metabolism and lipid peroxidation of a perennial grass Leymus chinensis.Planta,2006,4(5):1080-1090.
[31]	秦红杰,李敦海.铜绿微囊藻高温胁迫后的超补偿生长.环境科学,2010,1(7):1504-1509.QIN Hongjie,LI Dunhai.Over-compensatory growth of Microcystis aeruginosa after high temperature stress.Environmental Science,2010,1(7):1504-1509.
[32]	袁侃,毛献忠,陶益,等.UV-C辐照抑制铜绿微囊藻生长的动态试验研究.环境科学,2010,1(2):310-317.YUAN Kan,MAO Xianzhong,TAO Yi,et al.Dynamic experiment on controlling of Microcystis aeruginosa by UV-C irradiation.Environmental Science,2010,1(2):310-317.
[33]	SOHAL R S,ORR W C.The redox stress hypothesis of aging.Free Radical Biology and Medicine,2011,2(3):539-555.
[34]	IMAI H,CHANG K H,KUSABA M,et al.Temperature-dependent dominance of Microcystis (Cyanophyceae) species:M.aeruginosa and M.wesenbergii.Journal of Plankton Research,2009,1(2):171-178.
[35]	O'NEIL J M,DAVIS T W,BURFORD M A,et al.The rise of harmful cyanobacteria blooms:the potential roles of eutrophication and climate change.Harmful Algae,2011,4:313-334.