Abstract: To understand the influence of nitrate nitrogen (NO₃^--N) application on N₂O emissions from different soil types during the freezing process, a lab-simulated experiment was conducted to investigate N₂O production from black, fluvo-aquic and loess soils receiving different concentrations of NO₃^--N:control (0), 80,0 and 500 mg/kg soil. The application of NO₃^--N significantly increased N₂O emissions from the black and fluvo-aquic soils (P<0.05). Compared to the control treatment, the application of NO₃^--N with 200 mg/kg soil increased N₂O emissions from the black and fluvo-aquic soils by 849% and 676%, respectively. However, N₂O emissions from the 500 mg/kg NO₃^--N treatment soil were reduced by 39.3% and 21.2% compared to 200 mg/kg NO₃^--N treatment soil in the black and fluvo-aquic soils, respectively. As the freezing proceeded, the N₂O fluxes from the black and fluvo-aquic soils gradually approached zero. Although there was no significant N₂O flux found from the loess soil, N₂O uptake occurred frequently in the soil. Multi-factor variance analysis showed that soil pH and soil C-to-N ratio were the most important factors driving N₂O emissions. The results implied that management strategies aiming to mitigate N₂O emissions from soil should avoid NO₃^--N fertilizer application in black and fluvo-aquic soils during late autumn fertilization. Further studies to investigate the comprehensive relationship between N₂O emissions and variable field conditions involving climate, plants and soil conditions would be useful to expand this research.