Abstract: To determine the semi-volatile organic compounds (SVOCs) of river water samples from near Leptomitus lacteus growth locations, the SVOCs of spiked blank water samples extracted by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) pretreatment methods were analyzed and compared by gas chromatography and mass spectrometry with selective ion monitoring module (GC-MS/SIM). The SPE-GC-MS/SIM method, with good reproducibility, was selected to determine the SVOCs of the river water from the growing place of Leptomitus lacteus. The results showed that the spiked recovery of SVOCs extracted by LLE and SPE were 41.8%-121.5% and 31.5%-124.4% respectively, and the corresponding relative standard deviation (RSD) of SVOCs extracted by LLE and SPE were 6.0%-18.0% and 0.9%-14.5% respectively. The SVOC recovery by LLE was close to that by SPE, but the RSD of SPE was lower, which indicated that the reproducibility of SPE is better. Subsequently, the SPE GC-MS/SIM method was used to determine the SVOCs of the river water samples from the Leptomitus lacteus growth locations in February 2011, and 29 kinds of SVOCs were detected. The concentration of diethyl phthalate, which was the highest SVOC ingredient, was 1020.59 ng/L. The 1,4-dichlorobenzene, 1,2-dichlorobenzene, nitrobenzene, diethyl phthalate and polycyclic aromatic hydrocarbon concentrations were 19.57,8.52,3.39,0.59 and 532.14 ng/L respectively. All SVOC concentrations were lower than the limit values of the relevant indices in the National Regulation of Standards for Drinking Water Quality (GB 5749-2006), which illustrated that there was no SVOC contamination in the growth locations of Leptomitus lacteus by the river.