Abstract: The ecotoxicology database plays an important role in Chinese environmental protection and scientific research, particularly in the study of environmental criteria.At present, the lack of a basic toxicity database and platform supporting China's environmental management work has become one of the bottlenecks restricting the country in carrying out the creation of environmental standards, ecological risk assessment, environmental quality assessment, pollution control, toxicology and so on.Therefore, the establishment of a local biological toxicity database and platform is urgently needed in China.The technology and experience of the Ecotoxicology Knowledgebase (ECOTOX) are widely implied for the creation of similar databases in China.The present paper systematically summarized the core content of the ECOTOX's formation, namely literature collection, data sources, data acquisition and data collection elements, and compared the current domestic toxicity database and other databases closely related to the ecological survey data.Some problems exist in the ECOTOX, for instance, data entries (fields) still need to be increased further; the database construction is counter-intuitive; relatively few toxicity data have been collected in non-English literature; the connection with research related to ecotoxicology is not enough close; and the existing Chinese toxicity database lacks practicability and has poor reliability.From the perspectives of environmental standards, ecological risk assessment and toxicology research development, this paper proposes the creation of a Chinese ecological toxicity database, including complete, shared and effective ecological toxicity data resources; the formation of toxicological data processing standards and norms; the cohesion of existing domestic databases; and the use of large data cloud computing technology, with a focus on the collection of the local Chinese biology and toxicity data of new pollutants.