Abstract: The study of functional structure of aquatic communities not only forms the formation of river ecology, but also helps us to better understand the responses of aquatic organisms to environmental stressors. To evaluate the effects of land use on functional feeding groups (FFGs) structure, we investigated macroinvertebrate communities at 47 sampling sites (6 reference sites and 41 disturbed sites) and analyzed the relationships among the FFGs structure and watershed land use types (forest, grass, agricultural and urban land use) and water quality parameters in May 2009 in Taizi River Basin. The results showed that:(1) Kruskal-Wallis nonparametric test indicated that altitude (ALT), water temperature (WT), electrical conductivity (EC), ammonia nitrogen (NH₄⁺-N) concentration and phosphate (PO₄^3--P) concentration in disturbed sites were significantly higher than those in reference sites. (2) The relative abundance, species richness and density of predators, scrapers and shredders, species richness of collector-gatherers, Shannon-Wiener diversity index and Pielou evenness index of scrapers, Margalef richness index of collector-gatherers and predators and Simpson diversity index of scrapers in disturbed sites were significantly lower than those in reference sites, whereas the relative abundance of collect-gatherers in disturbed sites was higher than that in reference sites. (3) Spearman correlation analysis showed that increasing proportion of agricultural and urban land use led to the deterioration of water quality and the change in the FFGs structure of macroinvertebrate. (4) Canonical correspondence analysis (CCA) indicated that EC was the main environmental factor influencing the FFG structure characteristics of macroinvertebrate. The study showed that the FFGs of macroinvertebrate had a sensitive response to the development of agricultural activities and urbanization in Taizi River Basin, which provides theoretical support for monitoring environmental pressures based on the FFGs of macroinvertebrate in the future watershed management.