Abstract: Carbon sequestration of terrestrial ecosystem is one of the most important indicators in ecosystem service assessment and is crucial to accurate estimation of regional carbon budget, which could support decision-making for the regional carbon management. In the present study, we estimated the forest ecosystem carbon sequestration and analyzed its spatio-temporal variation in Xiamen City in 2015 by using VPM (Vegetation Photosynthesis Model) and ReRSM (Remote Sensing Model for Ecosystem Respiration). The result showed that the carbon sequestration of forest ecosystem in Xiamen City was 31.36×10⁴ t/a (measured on carbon) in 2015, with an average of 644.86 g/(m²·a). In general, the intra-annual dynamic of carbon sequestration exhibited a single peak curve, but fluctuated greatly under the influence of typhoons. The most areas of forest ecosystem in Xiamen City was carbon sink, with higher carbon sequestration at the edge of northwest and relative lower value in other regions, which was similar to the trend of DEM. At district scale, Tong'an District had the largest forest area and highest carbon sequestration, accounting for 52.58% and 57.10% of the total forest area and carbon sequestration in Xiamen, respectively. Tong'an District, coupled with Xiang'an and Jimei Districts made up 88.27% of the carbon sequestration of the whole city. Huli District was almost carbon neutral with an average carbon sequestration of 14.25 g/(m²·a). This research showed that the forest ecosystem in Xiamen City had a good carbon sequestration capacity.