Abstract: In order to explore the response patterns of macroinvertebrate to water quality indicators in different topographic zones of the Songhua River Basin and to identify the differences of indicator taxa for water quality parameters, water quality indicators (EC, ρ(DO), ρ(COD_Mn), ρ(NH₃-N), ρ(TN), ρ(TP)) and macroinvertebrate communities were investigated at 97 sampling sites from 2016 to 2018. The threshold indicator taxa analysis (TITAN) was used to analyze water quality indicator thresholds of macroinvertebrate community composition in mountainous, hilly and plain zones, respectively. When the pollutant concentration exceeded the negative thresholds, the sensitive taxa density decreased, whereas when the positive thresholds were exceeded, the tolerant species were also significantly affected, resulting in significant changes in the macroinvertebrate community structure. The negative thresholds were used as the minimum value to trigger the change of macroinvertebrate community, while the positive thresholds were used as the tolerance limit of macroinvertebrate community. The results showed that: (1) Except for ρ(DO) and ρ(COD_Mn), the negative thresholds of other water quality indicators showed a trend of mountain < hill < plain, while ρ(DO) was the opposite, and ρ(COD_Mn) had the highest threshold in hilly zone with the concentration of 5.46 mg/L, while the lowest (4.01 mg/L) in mountainous zone. (2) More than 50% sites exceeded the negative thresholds of water quality indicators, 5%-40% sites exceeded the positive thresholds, which means an extent of anthropogenic disturbance. (3) Some species in different topographic zones exhibited the opposite directions of water quality indicators. For example, Radix showed positive response to ρ(NH₃-N) in hilly zone, while in plain zone it turned to negative taxa. Semisulcospira exhibited positive response to ρ(TN) and ρ(TP) in hilly region, while in plain region it showed the opposite response. The study shows that physical-geography and habitat conditions in different zones are dominant factors influencing the distribution patterns of macroinvertebrate community, which indirectly affects indicator species screening in TITAN.