Abstract: Four kinds of vegetable including leaf lettuce, rape, pakchoi and lettuce were selected, the germination rate of seeds, the fresh weight of seedlings, shoot length and root length were used as the toxicity sensitive indicators, and the ecotoxicity response of penicillin (PG) between different treatment groups (10, 100, 200, 400, 800, 1200, 1600, 2000, 2400, 3200 and 4000 mg/L) and the control group to the germination of four vegetable seeds was studied to promote the establishment of technical specifications for the environmentally safe use of penicillin mycelial residues. The results showed that: (1) There was no significant difference in the germination rate of four vegetable seeds in the treatment group compared with in the control group(P>0.05); when the concentration of penicillin was higher than 1200 mg/L, the growth rate of shoot length of Rape seed was around 60% and there was a very significant difference compared with in the control group (P < 0.05); when the concentration of penicillin was 200 mg/L, the fresh weight of lettuce reached a maximum of 0.29 g, which increase 10.37% compared to in the control group, and pakchoi fresh weight reached a peak when the concentration of penicillin was 400 mg/L, an increase of 5.76% compared to in the control group. With the increase of penicillin concentration in the experimental concentration range, the root length inhibition rate in the treatment group increased. (2) The dose-response relationship between penicillin and the inhibition rate of root length of four vegetables was in accordance with the Allometric model: y=ax^b(where x is ρ(PG), y is the root length inhibition rate, a and b are constants). (3) The half inhibitory concentration (IC₅₀) of penicillin on the root length of leaf lettuce, rape, pakchoi and lettuce were 1675.11, 995.67, 3736.40, and 2510.24 mg/L, respectively, and the sensitivity of the root length inhibition rates of four kinds vegetable to penicillin was rape, leaf lettuce, lettuce, pakchoi in turn (P < 0.05). This study showed that rape was more suitable as an indicator plant of penicillin ecotoxicity compared with leaf lettuce, pakchoi and lettuce.