Abstract: Biofilter is the main method to treat odors in domestic waste treatment plant. One of the important factors affecting the performance of biofilter is the packing filler. It is known that compost derived from the fermentation of domestic waste can be used as packing filter. The compost product derived from the fermentation of domestic waste can be used as packing filter to achieve resource recycling to a certain extent. Aimed at the odors of toluene, methyl mercaptan, ammonia and hydrogen sulfide produced by domestic waste plant, biofilter simulated with cylindrical plexiglass was filled with compost obtained after 7 and 20 days of secondary fermentation. The removal efficiency of the odors, the changes of physical and chemical properties before and after deodorization were investigated, and the succession of microbial community in the packing filter with the compost product was studied. The results showed that the average removal efficiencies of ammonia, hydrogen sulfide, toluene and methyl mercaptan were all above 95%, and packing filter with compost product had the highest abundance of microbial community and removal rate of odors. The homology coefficient of the bacterial community in the early and late stages of operation was relatively high, and a stable biological community with the dominant species of Kineothrix, Bacillus and Weissella was formed. In conclusion, through the evaluation of odor removal efficiency, changes of physicochemical properties and microbial community of different compost fillers, it is found that the filler with compost product has the best odor removal effect, stable physicochemical properties and contains deodorizing bacteria, which can be used as packing filler of biofilter for deodorization in a domestic waste treatment plants.