Abstract: Surfactant-enhanced remediation technology is widely used in soil remediation. In this study, 11 types of non-ionic surfactants (including 5 non-ionic biosurfactants) and 3 ionic surfactants (2 anionic and 1 cationic biosurfactants) were selected for the remediation of soil contaminated with PAHs (phenanthrene, pyrene and benzoa pyrene). The surfactants with better elution effects were screened, and the effects of factors, such as surfactant concentration, elution time, solid-liquid ratio, and nonionic to anionic surfactant ratio, on the elution of PAHs in soil were further investigated in order to optimize the elution method. The results showed that non-ionic surfactants had a better elution effect on PAHs in soil than ionic surfactants. When the surfactant concentration was 10 g/L and solid-to-liquid ratio was 1∶20, the elution rate of polyoxyethylene ether -10 (NSF10) reached 78%, followed by Triton X-100 (76.7%) and Tween 80 (73.4%). The two anionic surfactants, sodium dodecyl sulfonate (SDS) and sodium dodecylbenzene sulfonate (SDBS), had an elution rate of less than 1% for PAHs in soil, which is ineffective. The elution rate of PAHs varied significantly with varying surfactant concentration. As the concentration increased, the elution rate increased linearly. When the concentration increased to 5 g/L, the elution rate increased rapidly. For example, the elution rate of NSF10 increased from 17.9% at 1 g/L to 60.4% at 5 g/L. The elution rate increased by 237.4% when it reached 10 g/L, and the elution rate increased by only 20.2% compared with 5 g/L. When the elution time was 16 h, the elution of PAHs by NSF10 reached equilibrium. When the elution time was extended, the elution effect was not enhanced. Increasing the volume of surfactants improved the elution of PAHs. The solid-liquid ratio of 1∶40 was the optimal solid-liquid ratio. At this ratio, the elution rate was 85.2% of that at a solid-liquid ratio of 1∶100. When non-ionic surfactants (NSF10, TX-100 and TW-80) and anionic surfactant SDS were mixed at a volume ratio of 9∶1, the elution effect was better than that of a single surfactant. When the volume ratio of NSF10∶SDS was 7∶3, the solubilization and elution effect were the most obvious, which was 18.2% higher than that of single surfactant. The results show that NSF10 was a high-efficiency eluent for PAHs, and the optimum synergistic elution effect was achieved at the concentration of 5 g/L, the elution time of 16 h and the solid-liquid ratio of 1∶40. It can achieve synergistic elution effect when NSF10 is mixed with SDS at the volume ratio of 7∶3.