Abstract: The border area of Jiangsu, Anhui, Shandong and Henan has become one of the key areas for national air pollution prevention and control in China. The aim of this study is to investigate the vertical structure characteristics of ozone, the influence of meteorological factors and potential sources in Jiangsu, Anhui, Shandong and Henan regions. The vertical structures of ozone and meteorology in Huaibei City in November 2021 were observed by lidar. The results showed that: (1) The surface ozone concentration was characterized by single-peak daily variation, and there was a continuous three-day high-altitude ozone pollution from November 16^th to 18^th, 2021. On the clean days (defined as the hourly high-altitude ozone concentration < 200 μg/m³), the ozone concentration below 1.5 km increased first and then decreased with the increase of altitude. On polluted days (defined as the hourly high-altitude ozone concentration ≥ 200 μg/m³), the ozone distribution appeared as double layers on November 16^th and 17^th, 2021 and the ozone concentration decreased with the increasing altitude below 1.5 km on the November 18^th. (2) At vertical altitude, the temperature on the polluted days was higher than that on the clean days, and the wind speed was lower than that on the clean days, indicating that high temperature and low wind were conducive to the generation of high-altitude ozone pollution. The inversion layer on the November 16^th played an important role in the accumulation of ozone concentration, and the changes in wind direction and downdraft were also the important causes of ozone accumulation in the upper air on the polluted days. (3) The analysis of potential ozone sources and their contributions showed that the high ozone concentration on the observation days (defined as the entire observation period: November 10^th-19^th, 2021) may be related to the local generation of Huaibei City and regional transport in the south and east of Huaibei City. The ozone concentration on the clean days was mainly affected by Henan Province. The high-altitude ozone pollution on the polluted days was related to the local generation and the transport of ozone and its precursors in surrounding cities. Based on the above research results, temperature, wind speed, wind direction and regional transport had significant effects on the evolution of the vertical structure of ozone concentration in Huaibei City.