Abstract: The research and development of biodegradable film is of great significance to alleviate the pollution of soil environment caused by traditional plastic mulch. Among different biodegradable materials, it is a new choice to use cellulose, hemicellulose and humic acid which are abundant in organic fertilizer to prepare biodegradable film, and the film-forming additives are the keys to prepare biodegradable films successfully by using organic fertilizers as the raw materials. In order to study the influence of film-forming additives on the membrane performance during film preparation, the mechanism of action of film-forming additives on organic fertilizer degradable films and the degradation pattern of the films were analyzed. In this study, sodium alginate, propanetriol, glutaraldehyde and sodium carboxymethylcellulose were used as film-forming additives, and the concentrations of film-forming additives were optimized in terms of tensile strength, elongation at break and gas permeability through orthogonal design tests. The results showed that the best overall performance of the films was achieved when the contents of sodium alginate, propanetriol, glutaraldehyde and sodium carboxymethylcellulose were 0.70% (mass percentage), 1.00%, 0.20% and 1.00%, respectively. The tensile strength of the optimized product was 16.88 MPa, and the corresponding break elongation was 28.47%. Furthermore, the ammonia and carbon dioxide permeability coefficients of the optimized product were 1.23×10⁻¹¹ and 1.03×10⁻¹¹ g·m/(m²·d·Pa), respectively. Spectral analysis results showed that the contents of functional groups such as hydroxyl, carboxyl, aldehyde groups and the crystallinity increased after the film was formed, resulting in a stable degradable film prepared from loose organic fertilizers. The soil simulation experiments showed that the degradation process of the degradable film conformed to the second-order degradation kinetic model, the degradation rate constant was 0.004, and the 32^nd day degradation weight loss was 58.97%, which is a good degradability. The present study shows that the addition of film-forming additives has a significant effect on the mechanical properties and gas permeability of the organic fertilizer degradation film, which has good degradation properties and practical value in agricultural production.