Abstract: From July to August 2022, the temperature in the Sichuan Basin was 3.63 ℃ higher than that of last year, and the number of ozone pollution days increased significantly compared to previous years. To understand the effects of high temperature on biogenic emissions and O₃ generation contribution in the Sichuan Basin, the Model of Emissions of Gases and Aerosols from Nature (MEGAN) was used to estimate the emissions of biogenic volatile organic compounds (BVOCs) in the Sichuan Basin from July to August 2022 based on the meteorological data obtained by Weather Research and Forecasting (WRF) simulations. The spatiotemporal distribution characteristics of BVOCs and chemical species were analyzed. The impact of BVOCs emissions on O₃ generation in high temperature weather was quantitatively evaluated by a Community Multi-Scale Air Quality (CMAQ) model. The results showed that: (1) From July to August 2022, BVOCs emissions in the Sichuan Basin were 86.30×10⁴ t, and the emissions of isoprene, methanol and monoterpenes were 42.16×10⁴, 17.32×10⁴ and 9.92×10⁴ t, respectively. The BVOCs emission intensities of Chengdu Plain urban agglomeration, South Sichuan urban agglomeration, and Northeast Sichuan urban agglomeration were 3.61, 6.55 and 5.00 t/km², respectively. (2) The high emission areas of BVOCs were mainly concentrated in the central parts of Yibin City and Luzhou City in the South Sichuan urban agglomeration, the northeastern part of Leshan City in the Chengdu Plain urban agglomeration, and the northern parts of Bazhong City and Dazhou City in the Northeastern Sichuan urban agglomeration. The spatial distribution characteristics of isoprene and monoterpene emissions were basically consistent with those of BVOCs emissions, while the spatial distribution characteristics of methanol showed significant differences. (3) BVOCs emissions had an obvious diurnal characteristic and the highest emission occurred at 14:00. (4) The CMAQ simulation results showed that the cities with large contributions of BVOCs emissions to O₃ generation were Leshan, Meishan, Ya′an, and Neijiang, with contribution rate of over 30%. In terms of spatial distribution, BVOCs emissions in the northern part of Leshan and the central-western parts of Meishan in Chengdu Plain urban agglomeration had the most significant contribution to O₃ generation. From July to August 2022, the contribution of BVOCs emissions to O₃ generation in high temperature weather was 4.99% higher than that of last year. Based on the above research results, BVOCs emissions in Sichuan Basin has an significant impact on O₃ pollution, and the contribution rate to O₃ generation further increases in high temperature weather, which is the focus for O₃ pollution prevention and control.