Abstract: In order to understand the status and development trends of lake nitrogen removal, the bibliometric software CiteSpace was used to analyze the researcher distribution, research status, hotspots, and development trends worldwide from 2000 to 2022 based on the Web of Science (WoS) and China National Knowledge Infrastructure (CNKI) database. The results show that: (1) The overall literature in the study field has been increasing and can be divided into two stages: mechanism exploration (2000-2010) and rapid development (2011-2022). (2) China has the highest number of publications and occupies a relatively central position in international scientific research cooperation. Many achievements in cooperation between domestic research institutions and scholars have been gained, however, international cooperation still needs to be strengthened. (3) The research on nitrogen removal involves multiple disciplines i.e., lake ecology, environmental chemistry, and environmental engineering. (4) In terms of research focus, both domestic and international attention has been paid to the nitrogen removal of shallow lakes and the microbial-mediated Anammox rate and denitrification rate, while China pays more attention to the synergistic treatment of lake eutrophication. The results of this study show that for future lake nitrogen pollution control, quantitative analysis of nitrogen removal capacity and driving factors in different lake areas and the exploration of nitrogen removal potential will be the research focus. The use of nitrogen stable isotope technology to track the sources and transformation of nitrogen in lakes of different ecological types, and the development of new biological nitrogen removal technologies to reduce nitrogen load and environmental risks still need to be further explored and verified.