Abstract: Establishing nitrogen and phosphorus nutritional criteria is crucial to preventing eutrophication of water bodies. This study screened the historical monitoring data of Hangzhou Bay from 2010 to 2022, used methods such as principal component analysis and correlation analysis to screent key nutrient criteria indicators, and applied comprehensive statistical methods such as frequency step method and pressure-response model, to analyze the indicators and obtain nutritional criteria. The research results show that: (1) Dissoloved inorganic nitrogen and reactive phosphate are key indicators in formulating Hangzhou Bay nutrient criteria and directly affect the eutrophication of water bodies and the stability of ecosystems. In addition, chlorophyll a and dissolved oxygen are important biological indicators for assessing the health of water bodies. (2) We observed significant differences in the nutrient criterion values derived from the frequency step method and the pressure-response model, with the latter yielding higher values. (3) Based on the current water quality and eutrophication level of Hangzhou Bay, nutrient criteria values were proposed (0.732 mg/L for dissoloved inorganic nitrogen and 0.033 mg/L for reactive phosphate). Comprehensive derivation of nutritional criteria values by multiple methods is more in line with the actual needs of aquatic ecological protection. The findings of this study provide scientific guidance for controlling eutrophication in Hangzhou Bay and formulating nutritional standards.