Abstract: Integrating biodiversity conservation into the development of various regions, departments, and industries, and establishing biodiversity conservation concepts across institutional, economic, social, and cultural dimensions are not only effective measure for biodiversity conservation and sustainable utilization, but also the consensus of policymakers and academia. The Convention on Biological Diversity (CBD) has consistently prioritized biodiversity mainstreaming. This article reviews the requirements of previous Conferences of the Parties (COP) decision and analyzes the outcomes, objectives, and visions of the 6th, 10th and 15th Conferences of the Parties. The analysis reveals that the CBD′s requirements for biodiversity mainstreaming have become more detailed, emphasizing the full participation of the governments and society. Based on this, a practical framework that includes political, economic, social, cultural, and environmental aspects is established. As an early signatory of the CBD, China places great importance to biodiversity mainstreaming. By continuously improving policy systems and integrating biodiversity protection across various fields, China strengthens the coordinated development of the economy, society, and biodiversity under the guidance of ecological civilization construction, promotes broad societal participation, and has made significant progress in biodiversity mainstream. It is recommended to systematically advance the construction of biodiversity regulatory and policy systems, promote synergy between biodiversity conservation and community development, enhance public participation in biodiversity conservation, focus on international cooperation and exchange, and continuously contribute China′s experience to the global biodiversity mainstreaming process.