Abstract: In this work, a strain of bacteria isolated form sewage and soil nearby coking plant has been reported. The strain uses phenol as sole carbon source and degrades phenol up to the concentration of 915mg/L. Optimal preparation condition of the strain immobilization is carried out by the cross-test experiments.Going further into the kinetic study of biodegradation for both free and immobilized cells proceeds in the simulated wastewater. Results obtained from degradation experiments agree fairly well with the Monod model. The final experimental results show that the degradation efficiency of immobilized cells is apparently superior to that of free cells.