Abstract: Fish of various species were collected from wastewater fishpond in Tianjin. Contents of copper and lead were measured for gill, liver, kidney and muscle of the fish. The metal speciation in the pond was calculated using chemical equilibrium modeling. It was found that the metal contents in the fish were lognormal distributed. Significant difference between various organs, rather than fish species, was revealed. Due to difference in kinetics of metal absorption and excretion, ratio of copper to lead decreased gradually, indicating that accumulation rate of lead declined during the second phase of the study period. The accumulation was positively correlated to the contents of bioavailable metal including hydrated and hydro complexed copper and lead.