Abstract: The advance in screening and testing assays for identifying endocrine-disrupting chemicals (EDCs) is reviewed.A tiered test program is recommended for prescreening,evaluation of the potential of endocrine disruption,and assessment of consequence of endocrine-disrupting potential of specific chemicals.The prescreening stage consists of automated assays to detect the binding affinity of chemicals with hormone receptors.Tier 1 involves a battery of in vitro and in vitro assays designed to detect the potential of a chemical to disrupt endocrine system.Tier 2 tests are designed to characterize the nature,likelihood,and dose-response relationship of endocrine disruption in humans and wildlife.A wide variety of SARs,especially QSARs,have been developed to predict the hormone binding affinity for the simple and inexpensive screening approaches.Great efforts are still needed for the development of validated and standardized program for screening and testing endocrine-disrupting effects of chemicals.