Abstract: The acute toxicity of Q67 luminescent bacteria( Vibrio-qinghaiensis sp.Nov. Q67)and the induction of aryl hydrocarbon hydroxylase(AHH)of carp liver microsome by antibiotic wastewater samples from the acidification sequence in one treatment was tested to evaluate the efficiency of acidification treatment to the wastewater.The results obtained demonstrated that besides the chemical analysis,the bioassay was essential to monitor the water quality.