Abstract: The biosorbent was prepared from the yeast of Beijing Beerhouse.The factors,including pH,initial concentration of Pb²⁺ and pretreatment of yeast with acid and alkali,which can influence the uptake capacity of the lead,were studied.The results showed that the uptake capacity of yeast increased with the increase of pH and reached its maximum at pH=6;the optional pH range was 4～7;increased initial Pb²⁺ concentration also resulted in an increased uptake;the highest uptake capacity of 107 mg/g was reached at initial Pb²⁺ concentration of 500 mg/L, pH=6.The adsorption pattern of Pb²⁺ on yeast can be well described via Langmuir isotherm.Pretreatment of the yeast cell with acid increased the amount of metal removal from the liquid phase.