木质素过氧化物酶和锰过氧化物酶对染料脱色的性能比较

Characterization Comparison of Lignin Peroxidase and Manganese Peroxidase on Decoloration of Dyes

摘要: 通过控制培养基中Mn²⁺的浓度获得只含有木质素过氧化物酶(LiP)或锰过氧化物酶(MnP)的单一酶培养物,用于比较LiP和MnP对染料降解脱色的条件和能力的差异.结果表明,LiP对橙Ⅰ降解脱色的最佳条件为h²O₂0.15 mmol/L,pH 3.0,温度40 ℃;MnP为h²O₂0.15 mmol/L,pH 3.5,温度40 ℃.最佳条件下,考察LiP和MnP作用下染料浓度和染料类型对脱色的影响,发现LiP作用下脱色率明显高于MnP作用下的脱色率,表明LiP可作用的染料浓度比MnP高,可作用的染料范围比MnP大.由此认为LiP具有比MnP更强的脱色能力.

Abstract: The cultures only containing lignin peroxidase (LiP) or manganese peroxidase (MnP) were obtained by controlling the concentration of Mn²⁺ in medium.The single enzyme culture was used to investigate the decoloration conditions and ability of LiP and MnP on dyes.The optimal decoloration condition of Orange Ⅰ by LiP was h²O₂0 15 mmol/L,pH 3.0 and temperature 40 ℃,while it was h²O₂0.15 mmol/L,pH 3 5 and temperature 40 ℃ by MnP.When the effect of concentration and type of dyes were studied under optimal conditions,higher decoloration was obtained when LiP was used.It was concluded that LiP could be applied in higher concentration and wider range of dyes than MnP could,which showed that the decoloration ability of LiP was much higher than that of MnP.