Abstract: Non-point sources pollution loading and its temporal and spatial variability were discussed through the integration of trial experiments,GIS techniques and pollution models in Suzhou creek.It was found that non-point sources became the main pollution sources,and annual pollution loading of COD_Cr reached about 6 740 t,and TN,TP and NH₄⁺-N about 914,184 and 298 t respectively in the study area.Rice paddy fields and villages were the major contributors of pollution,and various non-point sources pollution loading were all in the same and obvious seasonal rule:summer rain>autumn rain>spring rain>winter rain.