Abstract: The comet assay was used to detect the DNA damage of spleen cells in mice,which were induced by 12 tested hydroxybenzene compounds.The results showed that all the 12 tested substances could induce different levels of DNA damage.The dose-response relations were obvious.Compared with the control group,all the treated groups were significantly different (P<0.05,P<0.01).It was also shown that the degree of DNA damages was partly related to the structure of hydroxybenzene compounds.