Abstract: From the sludge of a chemical plant,12 strains of tetrahydrofuran degrading bacteria were screened and all identified as Aeromonas kluger and \%Van Niel\%.The plasmid\|carrying rate was 100% by alkaline lysis method.In laboratory situation,the maximal tolerance of strains could reach at 140 μL/mL.All of the bacteria showed fastness to Streptomycin Sulfate,some showed fastness to Ampicillin Sodium and none showed fastness to Amikacin Sulfate,Gentamicin Sulfate and Chloramphenicol.In mineral medium(MM) and with tetrahydrofuran as exclusive carbohydrate,shaking cultured at 30 ℃ and 108 r/min in rotary vibrator for 6 d,tetrahydrofuran degrading strains 1,2,4 and 9 displayed relatively high degrading ability.Strain 1 and strain 9 were confirmed for more research.The optimal temperature for these two strains was 30 ℃ and the optimal pH was 7.5.