Abstract: A method to measure urinary metabolites of polycyclic aromatic hydrocarbons(PAHs) was established based on experiments.The method involves enzymatic hydrolysis of PAHs metabolites and HPLC-fluorescence detection of three hydrolysis products,i.e. 1-OH-pyrene,9-OH-benzoa pyrene and 3-OH-benzoapyrene.The stability of these metabolites under different storage temperature was also examined.The results from the analysis of 200 human urine samples preliminarily confirmed the feasibility of this method.Recovery rate of these metabolites by this method is 70%～85%.Method detection limit is 0.02～0.05 μg/L.Conentration of these three metabolites is degraded by 10%～36% when stored at-20　℃ after one month.Stability in urine extract is better than in original urine.Higher storage temperature may cause worse stability.