Abstract: Main kinetic parameters in anaerobic granular sludge fluidized bed(AGSFB) process and their determination were advanced.The anaerobic kinetic coefficient was determined by batch test.The thickness of microbe methane produce layer in the Two-layer Model of granular sludge was calculated by equilibrium equation of substance metabolism.The diameter and dry density of granular sludge were tested,and the diffusion coefficient also confirmed.The calculation results of the kinetic model based on all the above parameters agreed with the long-term running status of the reactor,which proved the reliabilities of the tested parameters and the kinetic model.