Abstract: A method for the determination of eight phthalic acid esters of environmental hormone in water samples by using solid-phase extraction on HLB cartridge and reversed-phase liquid chromatography was established. Several factors that would affect recoveries of analytes were optimized by orthogonal design. The optimized results were as follows: eluting solvent was V(methanol)∶V(ethyl ether)=1∶19, eluting rate 1.0 mL/min, elution volume 9 mL, and washing solvent was V(methanol)∶V(H2O)=1∶19. Linear correlation coefficient (r) was over 0.999. The limits of detection were between 0.10 and 0.62 μg/L. The recoveries of eight phthalic acid esters were between 75.1% and 115.5%. The repeatability expressed as relative standard deviation was between 0.9% and 2.2%. The method was applied to analyze phthalic acid esters of environmental hormone in wastewater. Four kinds of phthalic acid esters (DEP, BBP, DBP and DEHP) were checked out.