Abstract: Arsenic can be transformed into volatilizable arsenic by microorganism in the soil and transferred to atmosphere. The authors focused on the effect of different environmental conditions on the biovolatilization of arsenic, and the results showed that the velocity of biovolatilization increased with the increase of arsenic concentration in the soil. Organic fertilizer could accelerate the biovolatilization of arsenic and the moisture content in the soil also affected the biovolatilization of arsenic. The velocity of biovolatilization became lower if the moisture was high enough or low enough in the soil.