Abstract: Hydroponic cultures were conducted to investigate the effects of EDTA on plant growth and Pb accumulation by seedlings of two Brassica juncea Czern.et Coss varieties under Pb stress. The results showed that 0.5 and 1.0 mmol/L Pb or EDTA treatments resulted in a dramatic inhibition of seedlings growth, while EDTA and Pb complex treatments decreased phytotoxicity of Pb or EDTA. Compared to the control (without addition of Pb and (EDTA),) there was no distinct diversity in plant growth under equimolar concentrations of Pb and (EDTA) treatments. These results showed that the phytotoxicity of seedlings mainly come from free Pb2+ or EDTA2-, and the chelated Pb-(EDTA) may have no or little phytotoxicity. EDTA increased Pb translocation from roots to shoots, and increased Pb accumulation in shoots. The maximum total Pb accumulation by plant shoots occurred when the concentration ratio of EDTA to Pb is equal to 1∶2. With increasing levels of EDTA, the accumulation of Pb in roots decreased. The reactions of (EDTA) treatments on Pb accumulation by two varieties were extremely similar, while the higher biomass variety had the higher effect on total Pb accumulation.