Abstract: To avoid using aluminum-bearing material, activated clinoptilolite wasmodified by Fe³⁺ ion. Static and dynamic experiments were carried out to study the defluoridation characteristics and impact of physio-chemical factors. The results indicated that the Fe³⁺-modification significantly increased defluoridation capacity (DC) of clinoptilolite. The static DC and dynamic DC were0.665 and 0.2 mg/g, respectively, corresponding to twenty times and seven times of that of original clinoptilolite (0.03 mg/g). The optimal pH range is 6～7. The adsorption fits in Langmuir model. Fluoride adsorption by Fe³⁺-carried clinoptilolite is fast, and the adsorption capacity was stable after regeneration. Fe³⁺-modified clinoptilolite also showed capacity to remove manganese from drinking water; the manganese adsorption capacity (MRC) was 2.442 mg/g. The result of treatment experiment with high-fluoride underground water showed that the water qualityafter treatment met the national criteria of drinking water. The safety and effectiveness in fluoride and manganese removal indicate that Fe³⁺-modified clinoptilolite is competitive in market of defluoridation techniques of drinking water.