Abstract: Dynamic adsorption of inactive-immobilized-discarded Saccharomyces cerevisiae on Pb²⁺ in continuous_upflow reactor was studied through orthogonal experiment to investigate the optimal operating parameters as well as affecting factors, such as pH, flow rate, filling density of yeast on adsorption effectiveness. It showed that the optimal conditions were of pH 6, flow rate 5 mL/min, and filling density of yeast 80 g/L. In the early 40 minutes of the experiment, C_t/C increased fast, which indicated that a majority (up to 92%) of Pb²⁺ was adsorbed. The adsorption process by inactive-immobilized discarded Saccharomyces cerevisiae in continuous_upflow reactor was very fast. It was feasible to wipe off Pb²⁺in wastewater by continuous_upflow reactor.