Abstract: Experimental design plays an important role in the study of mixture toxicity. Using the microplate toxicity test method, luminescence inhibition toxicity of seven nitrobenzene derivatives (NBDs) to Vibrio qinghaiensis sp.-Q67was determined. The-lg EC₅₀ values (EC₅₀ unit: mol/L) of sevenNBDs, i.e. nitrobenzene, 1-chloro-2-nitrobenzene, 1-chloro-3-nitrobenzene, 1-chloro-4-nitrobenzene, 3-nitroaniline, 4-nitroaniline and 4-Nitrotoluene, are 2.66, 3.22, 3.30, 3.29, 2.94, 4.22 and 3.39, respectively. On the basis of the dose-response relationships of seven NBDs, the uniform design was used to construct 10 NBD mixtures with different effect concentration. Similarly, applying microplate toxicity test method the toxicities of 10 NBD mixtures to Q67 were determined. The models for evaluating and predicting the mixture toxicity were developed using the dose additionand independent action principles. The results showed that compared to the equivalent-effect concentration ratio method, the concentration ratios of various NBD components in the mixtures constructed by the uniform design were scattered over the three-dimensional concentration space, which can examine the possible mixture types in a greater scope, closer to the actual environmental system.