Abstract: The distribution and transportation of polycyclic aromatic hydrocarbon (PAH) contamination in soil under sulfate-reducing conditions were studied through column experimentation.Phenanthrene，anthracene and pyrene were selected as representative PAHs，and their vertical distributions in the soil column，their mass concentrations，and sulfate radical ion and E_h values in leaching solution weredetected under simulated sulfate-reducing conditions.Also，the mass balance of PAHswas analyzed to calculate the biodegradation rate of PAHs.The result showed that the leaching ratios of phenanthrene and anthracene were not more than 2%，andtheir residual ratios after 120 days were 51.87% and 51.21%，respectively.However，more than 90% of pyrene was absorbed in the soil and not detected in theleaching solution.The concentrations of sulfate radical ion and E_h of theleaching solution decreased with leaching time; this was attributed to O₂ depletion.Phenanthrene，anthracene and pyrene degraded under sulfate-reducing conditions，and，after 120 days，their biodegradation ratios were 47.41%，48.10% and 3.61%，respectively.Pyrene degradation was slowest.The experiment result indicated that phenanthrene，anthracene and pyrene could be degraded by acclimated native microorganisms in soil under sulfate-reducing conditions，which provided a theoretical basis for in-situ remediation of PAHs pollution in soil.