Abstract: The performance of a hybrid upflow anaerobic sludge blanket reactor (UASB)-membrane bioreactor (MBR) system for berberine wastewater treatment was investigated.The carbon source was a mixture of berberine hydrochloric and glucose.The berberine hydrochloric in the wastewater was removed through synergistic biodegradation with glucose as the primary energy source.For different berberine hydrochloric proportions from 7.5% to 25.0% share of all ρ(COD_Cr),HRT was 24 h,the influent ρ(COD_Cr),ρ(NH₄⁺-N) and ρ(berberine hydrochloric) were 1717-4393,91.8-158.7 and 64.4-276.8 mg/L,respectively;the overall removal efficiency of the system was 92.5%-95.9% for ρ(COD_Cr),67.0%-98.9% for ρ(NH₄⁺-N) and more than 99% for ρ(berberine hydrochloric).Most berberine hydrochloric was removed in UASB with the removal rate of 95.2%-98.9%.At organic load rates from 0.54 to 1.88 kg COD_Cr/(m³·d) and berberine hydrochloric load rates from 0.71 to 12.42 g/(m³·d),the MBR ensured the average effluent ρ(COD_Cr),ρ(berberine hydrochloric) and ρ(NH₄⁺-N) were lower than 50,1.0 and 2.0 mg/L,respectively.With the increase of influent ρ(berberine hydrochloric) to 3.45-12.42 mg/L,under microbe toxicity stress conditions from berberine hydrochloric,a morphology change of activate sludge from flocs to granules was observed in the MBR.