Abstract: In order to understand the environmental behavior of antibiotics on agriculture soil in South China, a typical veterinary drug, tylosin, was investigated using batch experiments to determine its sorption kinetic and thermodynamic properties on the four typical agricultural soils in South China. The results showed that the sorption of tylosin was negatively correlated with the pH of the soil, with no significant correlation with organic matter content. A pseudo second-order rate equation could fit the sorption data well, indicating that the sorption rate might be controlled by intra-particle diffusion process. Sorption was a spontaneous and endothermic process. This study suggests that tylosin tends to remain in the agriculture soil of south subtropical regions, and its potential environmental risk should be considered.