Abstract: A mass of pharmaceutical waste is produced during antibiotic production by fermentation.The resource utilization of pharmaceutical waste is restricted greatly because of the existence of antibiotic residue.The aim of this study was to investigate a microbiological method to degrade tylosin residue in pharmaceutical waste.The results showed that tylosin-degrading bacteria were screened from the soil on which the tylosin pharmaceutical waste was long stacked.Tylosin was not detected in the pharmaceutical waste after fermentation with the tylosin-degrading bacteria for 120 h with conditions of initial bacteria concentration 10%,temperature 30-35 ℃,and pH 7.This indicates that tylosin residue in pharmaceutical waste can be eliminated by using the microorganism.The results obtained from the present study provide a reference method for treatment of tylosin pharmaceutical waste.