Abstract: The concentrations of seven heavy metals (Cu, Mn, Zn, Fe, Ni, Cd and Pb) were measured by WFX-110 atomic absorption spectrophotometry in different tissues of economic aquatic animals from sampling points of the Bengbu segment of the Huaihe River.The results showed that the concentrations of the seven heavy metals were different in the same tissues of different animals.The concentrations of Fe, Zn, Cu, Mn and Ni were higher than those of Cd and Pb.The variation tendency of heavy metals was in the order of Macrobrachium nipponense>A.woodiana woodiana>fishes.The content sequence of the seven heavy metals in fish and shellfish was Fe>Mn>Zn>Ni>Cu>Pb>Cd.Cu, Mn, Zn and Fe were mainly accumulated in the intestines, gills, hepatopancreas, etc., but were lower in the testes, muscles, etc.tissue of the fish and shellfish.The concentrations of Ni, Cd and Pb were higher in the hepatopancreas, gills, etc., but lower in the muscles, brain and other tissues of the tested animals.Enrichment of heavy metals in Macrobrachium nipponease and A.woodiana woodiana was obviously higher than that in the freshwater fish.Gills and hepatopancresa were the major organs of heavy metals concentration in the fish and shellfish.The concentrations of Cd and Pb in the edible parts of economic aquatic animals from the Bengbu segment of the Huaihe River were both higher than the China′s agricultral industry standard and the Health Standard of Consumption, therefore the edible parts of the animal were unfit for human consumption.The present results indicate that the organisms in sampling points of the Bengbu segment of the Huaihe River were significantly polluted by heavy metals.