Abstract: Parallel factor analysis was used to extract the fingerprint features of the Excitation-Emission Matrix Spectrum of dissolved organic matter in water collected from 13 sampling sites in the Chongqing Section of the Jialing River in June 2010.Five separate fluorescent components in four categories were identified,including two fulic-like components(C1:250,320 nm/400 nm and C4:230,340 nm/450 nm),one humic-like component(C2:260,360 nm/450 nm),one component produced by phytoplankton(C3:260 nm/380 nm) and one protein-like component(C5:280 nm/330 nm).The contribution rates of each category to the total fluorescence intensity were 18%-38%,11%-18%,8%-22% and 33%-49%,respectively.The DOM in the Chongqing Section of Jialing River showed a predominantly terrestrial origin,coming from industry,domestic wastewater,and other pollution caused by human activities.Metabolism of the phytoplankton contributed little to the water DOM.The total fluorescence intensity in the 13 sampling sites showed a zigzag trend in spatial distribution.