Abstract: Some bacteria were isolated from soil and sewage treatment systems by adding a resuscitation promoting factor(Rpf) to the culture medium.From these bacteria,four strains whose flocculation efficiency was higher than 50% were selected by adopting Kaolin suspension as the active evaluation system.Through a culture operation,multiple colonies of M3+M7 with high flocculation efficiency were constructed.Its flocculation efficiency could reach 96.27% under optimal conditions.The crude bioflocculant MAC37 was obtained by purification and solidification of the fermentation liquid cultured under optimized conditions,and the application of multiple microorganisms fermentation in adhesive wastewater treatment was studied.Qualitative and quantitative analysis showed that the main components of MAC37 were polysaccharides(74.5%) and proteins(20.4%).The removal rate of turbidity,color and CODCr of the adhesive wastewater were 92.57%,94.73% and 92.12%,respectively,after the treatment by the mltiple fermentation.